Analysis of pina and pinb alleles in the micro-core collections of chinese wheat germplasm by ecotilling and identification of a novel pinb allele
文献类型：期刊论文
| 作者 | Wang, Jing1,2; Sun, Jiazhu1; Liu, Dongcheng1; Yang, Wenlong1; Wang, Daowen1; Tong, Yiping1; Zhang, Aimin1 |
| 刊名 | Journal of cereal science
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 48期号:3页码:836-842 |
| ISSN号 | 0733-5210 |
| 关键词 | Kernel hardness Puroindoline allele Ecotilling Chinese wheat germplasm |
| DOI | 10.1016/j.jcs.2008.06.005 |
| 通讯作者 | Tong, yiping(yptong@genetics.ac.cn) |
| 英文摘要 | Kernel hardness is mainly conditioned by allelic variations of pina-d1 and pinb-d1 genes located on the short arm of chromosome 5d. in this work, the ecotilling approach was optimized to investigate pina and pinb alleles in the micro-core collections of chinese wheat germplasm, and three pina and eight pinb alleles were found. generally, more no alleles were detected in the accessions coming from the regions that grow winter or a mixture of spring and winter wheats. this was particularly evident for the southwestern winter wheat, xinjiang winter-spring wheat and yellow and huai river valley winter wheat regions. a novel variant (designated as pinb-d1x) was discovered in one of the accessions from the xinjiang winter-spring wheat region. compared to wild type (wt) allele pinb-d1a, two nucleotide substitutions occurred in the coding region of pinb-d1x, one (at nucleotide position 257) resulting in the replacement of a wt cysteine residue by tyrosine and the other (at nucleotide position 382) creating a premature stop codon. the implications of our data to understanding the diversity of pina and pinb alleles in wheat and to future molecular breeding of wheat kernel hardness are discussed. (c) 2008 elsevier ltd. all rights reserved. |
| WOS关键词 | PUROINDOLINE-B ; GRAIN HARDNESS ; MOLECULAR-GENETICS ; WINTER WHEATS ; SPRING WHEAT ; GENES ; CULTIVARS ; DIVERSITY ; MUTATION ; POPULATIONS |
| WOS研究方向 | Food Science & Technology |
| WOS类目 | Food Science & Technology |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000261087300039 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2392696 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tong, Yiping |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Sun, Jiazhu,Liu, Dongcheng,et al. Analysis of pina and pinb alleles in the micro-core collections of chinese wheat germplasm by ecotilling and identification of a novel pinb allele[J]. Journal of cereal science,2008,48(3):836-842. |
| APA | Wang, Jing.,Sun, Jiazhu.,Liu, Dongcheng.,Yang, Wenlong.,Wang, Daowen.,...&Zhang, Aimin.(2008).Analysis of pina and pinb alleles in the micro-core collections of chinese wheat germplasm by ecotilling and identification of a novel pinb allele.Journal of cereal science,48(3),836-842. |
| MLA | Wang, Jing,et al."Analysis of pina and pinb alleles in the micro-core collections of chinese wheat germplasm by ecotilling and identification of a novel pinb allele".Journal of cereal science 48.3(2008):836-842. |
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来源：中国科学院大学
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