Investigation on the interaction between a heterocyclic aminal derivative, sbdc, and human serum albumin
文献类型：期刊论文
| 作者 | Zhou, Qiuju1,2; Xiang, Junfeng1; Tang, Yalin1; Liao, Jiangpeng2,3; Yu, Chuyi3; Zhang, Hong1; Li, Lin1; Yang, Yueyang1; Xu, Guangzhi1 |
| 刊名 | Colloids and surfaces b-biointerfaces
 |
| 出版日期 | 2008-01-15 |
| 卷号 | 61期号:1页码:75-80 |
| 关键词 | Heterocyclic ketene aminals Human serum albumin Fluorescence quenching Circular dichroism Protein-ligand docking |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2007.07.007 |
| 通讯作者 | Tang, yalin(tangyl@iccas.ac.cn) |
| 英文摘要 | The interaction between a novel promising drug (spiro[(2r,3r,4s)-4-benzyloxy-2,3-isopropylidene-dioxy-1-oxa-cyclopentane-5,5'-(2-benzoylmethylene-1,3-diaza-cyclohexane)] (sbdc)) and human serum albumin (hsa) under physiological conditions has been investigated by using fluorescence, absorption, and circular dichroism (cd) spectroscopic techniques in combination with protein-ligand docking study. it was observed that sbdc has a strong ability to quench the intrinsic fluorescence of hsa through a static quenching procedure. the association constants of sbdc with hsa were determined at different temperatures based on fluorescence quenching results. the negative delta h and positive delta s values in case of sbdc-hsa complex showed that apart from an initial hydrophobic association, both van der waals interactions and hydrogen bonding play a vital role in the binding of sbdc to hsa. the quantitative analysis data of cd spectra showed that the binding of sbdc to hsa induced conformational changes in hsa and the alpha-helix of 52.1% in free hsa increased to 55.7% in hsa-sbdc complex. the distance between donor (hsa) and acceptor (sbdc) was obtained according to the forster's theory of non-radiation energy transfer. data obtained by spectroscopic techniques and protein-ligand docking study suggested that sbdc binds to residues located in subdomain iia of hsa. (c) 2007 elsevier b.v. all rights reserved. |
| WOS关键词 | KETENE AMINALS ; FUSED DIAZAHETEROCYCLES ; ETHYL BROMOACETATE ; OCHRATOXIN-A ; BINDING-SITE ; FLUORESCENCE ; PROTEIN ; CYCLOCONDENSATION ; ALKYLATION ; ROUTE |
| WOS研究方向 | Biophysics ; Chemistry ; Materials Science |
| WOS类目 | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000252584300011 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2392824 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tang, Yalin |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci BNLMS, Ctr Mol Sci,State Key Lab Struct Chem Unstable &, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Chem, Lab Mol Recognit & Select Synth, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qiuju,Xiang, Junfeng,Tang, Yalin,et al. Investigation on the interaction between a heterocyclic aminal derivative, sbdc, and human serum albumin[J]. Colloids and surfaces b-biointerfaces,2008,61(1):75-80. |
| APA | Zhou, Qiuju.,Xiang, Junfeng.,Tang, Yalin.,Liao, Jiangpeng.,Yu, Chuyi.,...&Xu, Guangzhi.(2008).Investigation on the interaction between a heterocyclic aminal derivative, sbdc, and human serum albumin.Colloids and surfaces b-biointerfaces,61(1),75-80. |
| MLA | Zhou, Qiuju,et al."Investigation on the interaction between a heterocyclic aminal derivative, sbdc, and human serum albumin".Colloids and surfaces b-biointerfaces 61.1(2008):75-80. |
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来源：中国科学院大学
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