Indian mustard aquaporin improves drought and heavy-metal resistance in tobacco
文献类型：期刊论文
| 作者 | Zhang, Yuxiu1; Wang, Zi1; Chai, Tuanyao2; Wen, Zhensong1; Zhang, Hongmei1 |
| 刊名 | Molecular biotechnology
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 40期号:3页码:280-292 |
| 关键词 | Aquaporin Drought Heavy metal Tobacco Indian mustard |
| ISSN号 | 1073-6085 |
| DOI | 10.1007/s12033-008-9084-1 |
| 通讯作者 | Zhang, yuxiu(zhangyuxiu@cumtb.edu.cn) |
| 英文摘要 | An aquaporin cdna bjpip1 isolated from heavy-metal accumulator indian mustard (brassica juncea l.) encodes a 286-residue protein. the deduced amino acid sequence of bjpip1 with six putative transmembrane domains showed highest identity (85-99%) to pip1 subfamily members. semi-quantitative rt-pcr analysis revealed that bjpip1 transcripts were more abundantly expressed in roots compared to aerial parts of indian mustard. however, the expression of bjpip1 in leaves was up-regulated by drought, salt, low temperature, and heavy metal stress, suggesting that bjpip1 was involved in resistance to abiotic stresses. bjpip1 under the control of 35s promoter was introduced into tobacco mediated with agrobacterium tumefaciens, the transgenic tobacco exhibited a lower water loss rate, a decreased transpiration rate, and stomatal conductance compared to the wild-type plants under osmotic stress, indicating that bjpip1 might enhance plant drought resistance by decreasing transpiration via reducing stomatal conductance. furthermore, overexpression of bjpip1 in tobacco enhanced cd resistance of root growth, and lowered transpiration rate and stomatal conductance upon cd exposure, suggesting that bjpip1 might increase heavy-metal resistance by maintaining reasonable water status in tobacco. moreover, the bjpip1-overexpressing plants showed higher activities of antioxidative enzymes, and lower level of electrolyte leakage and malondialdehyde content under cd stress, indicating bjpip1 might enhance the antioxidative activity and membrane integrity in transgenic plants. taken together, these results suggested that bjpip1 might improve plant heavy-metal resistance through alleviating water deficit and oxidative damage induced by metal ions. |
| WOS关键词 | PLASMA-MEMBRANE AQUAPORINS ; MONITORING EXPRESSION PROFILES ; TRANSGENIC RICE PLANTS ; PHASEOLUS-VULGARIS ; GENE FAMILY ; PHYSIOLOGICAL FUNCTIONS ; SUPEROXIDE-DISMUTASE ; LIPID-PEROXIDATION ; HYDROGEN-PEROXIDE ; COLD-ACCLIMATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000260068300008 |
| 出版者 | HUMANA PRESS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393186 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Yuxiu |
| 作者单位 | 1.China Univ Min & Technol Beijing, Sch Chem & Environm Engn, Dept Bioengn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuxiu,Wang, Zi,Chai, Tuanyao,et al. Indian mustard aquaporin improves drought and heavy-metal resistance in tobacco[J]. Molecular biotechnology,2008,40(3):280-292. |
| APA | Zhang, Yuxiu,Wang, Zi,Chai, Tuanyao,Wen, Zhensong,&Zhang, Hongmei.(2008).Indian mustard aquaporin improves drought and heavy-metal resistance in tobacco.Molecular biotechnology,40(3),280-292. |
| MLA | Zhang, Yuxiu,et al."Indian mustard aquaporin improves drought and heavy-metal resistance in tobacco".Molecular biotechnology 40.3(2008):280-292. |
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来源：中国科学院大学
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