Trichostatin a and nuclear reprogramming of cloned rabbit embryos
文献类型：期刊论文
| 作者 | Shi, L. H.1,2; Ai, J. S.1,2; OuYang, Y. C.1; Huang, J. C.1,2; Lei, Z. L.1,2; Wang, Q.1,2; Yin, S.1,2; Han, Z. M.1; Sun, Q. Y.1; Chen, D. Y.1 |
| 刊名 | Journal of animal science
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 86期号:5页码:1106-1113 |
| ISSN号 | 0021-8812 |
| 关键词 | Histone acetylation Rabbit fertilized embryos Somatic cell nuclear transfer embryos Trichostatin a |
| DOI | 10.2527/jas.2007-0718 |
| 通讯作者 | Chen, d. y.(chendy@ioz.ac.cn) |
| 英文摘要 | To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, trichostatin a (tsa). in the present study, global changes in acetylation of histone h3-lysine 14, histone h4-lysine 12, and histone h4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (scnt) embryos, and tsa-treated scnt embryos. from the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone h3-lysine 14 and histone h4-lysine 12 occurred in both fertilized embryos and tsa-key treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. more interesting, the signal of acetylation of histone h4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas tsa-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. the histone acetylation pattern of tsa-treated scnt embryos appeared to be more similar to that of normal embryos, indicating that tsa could improve nuclear reprogramming after nuclear transfer. |
| WOS关键词 | HISTONE ACETYLATION ; EPIGENETIC CHARACTERISTICS ; PREIMPLANTATION EMBRYOS ; OOCYTE MEIOSIS ; MOUSE CLONING ; DONOR CELLS ; METHYLATION ; DNA ; TRANSCRIPTION ; MICE |
| WOS研究方向 | Agriculture |
| WOS类目 | Agriculture, Dairy & Animal Science |
| 语种 | 英语 |
| 出版者 | AMER SOC ANIMAL SCIENCE |
| WOS记录号 | WOS:000255025500011 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393255 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, D. Y. |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, L. H.,Ai, J. S.,OuYang, Y. C.,et al. Trichostatin a and nuclear reprogramming of cloned rabbit embryos[J]. Journal of animal science,2008,86(5):1106-1113. |
| APA | Shi, L. H..,Ai, J. S..,OuYang, Y. C..,Huang, J. C..,Lei, Z. L..,...&Chen, D. Y..(2008).Trichostatin a and nuclear reprogramming of cloned rabbit embryos.Journal of animal science,86(5),1106-1113. |
| MLA | Shi, L. H.,et al."Trichostatin a and nuclear reprogramming of cloned rabbit embryos".Journal of animal science 86.5(2008):1106-1113. |
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来源：中国科学院大学
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