Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions
文献类型：期刊论文
| 作者 | Li, Chun-Hong1,2; Yu, Nan1,2; Jiang, Shi-Min1,2; Shangguan, Xiao-Xia1,2; Wang, Ling-Jian1; Chen, Xiao-Ya1 |
| 刊名 | Planta
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 228期号:1页码:125-136 |
| 关键词 | Arabidopsis Cytokinin Dna methylation S-adenosyl-l-homocysteine hydrolase |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-008-0724-2 |
| 通讯作者 | Chen, xiao-ya(xychen@sibs.ac.cn) |
| 英文摘要 | S-adenosyl-l-homocysteine hydrolase (sahh) is a key enzyme for maintenance of cellular transmethylation potential. although a cytokinin-binding activity had been hypothesized for sahh, the relation between cytokinin and transmethylation reactions has not been elucidated. here we show that, of the two arabidopsis thaliana sahh genes, atsahh1 has a much higher expression level than atsahh2. a t-dna insertion mutant of atsahh1 (sahh1-1) and the rna interference (rnai) plants (dsatsahh2) accumulated a higher level of cytokinins, exhibited phenotypic changes similar to those of cytokinin-overproducers, and their global dna methylation status was reduced. on the other hand, cytokinins positively regulate the transmethylation pathway genes, including atsahh1, atadk1 (for adenosine kinase), and this regulation involves the cytokinin activity. furthermore, expression of three cytosine dna methyltransferase genes examined was inducible by cytokinin treatment. unlike adenine and adenosine which are sahh inhibitors, the adenine-type cytokinins have no effect on sahh activity at protein level. changing of endogenous cytokinin levels by transgene expression resulted in alterations of dna methylation status in the sahh1-1 background, suggesting that cytokinins promote dna methylation, at least under transmethylation stringent conditions. these data demonstrate that the phytohormone cytokinin plays a role in promoting transmethylation reactions, including dna methylation. |
| WOS关键词 | DNA METHYLATION ; ADENOSYLHOMOCYSTEINE HYDROLASE ; ARABIDOPSIS-THALIANA ; PLANT DEVELOPMENT ; BINDING PROTEIN ; LEAF SENESCENCE ; NEPLANOCIN-A ; GENE ; TOBACCO ; HYPOMETHYLATION |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000256083200011 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393281 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Xiao-Ya |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chun-Hong,Yu, Nan,Jiang, Shi-Min,et al. Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions[J]. Planta,2008,228(1):125-136. |
| APA | Li, Chun-Hong,Yu, Nan,Jiang, Shi-Min,Shangguan, Xiao-Xia,Wang, Ling-Jian,&Chen, Xiao-Ya.(2008).Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions.Planta,228(1),125-136. |
| MLA | Li, Chun-Hong,et al."Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions".Planta 228.1(2008):125-136. |
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来源：中国科学院大学
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