中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis

文献类型:期刊论文

作者Tian Yi1,2,3,4; Kong Jie1; Wang WeiJi1
刊名Chinese science bulletin
出版日期2008-04-01
卷号53期号:8页码:1205-1216
关键词Aflp Fenneropaeneus chinensis Genetic linkage maps
ISSN号1001-6538
DOI10.1007/s11434-008-0170-3
通讯作者Kong jie()
英文摘要Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in china. a genetic linkage map is essential for improving the efficiency of its breeding by marker-assisted selection and identifying commercially important genes. linkage maps of f chinensis were constructed with an f2 mapping population (110 progenies) using amplified fragment length polymorphic (aflp) marker in this study. fifty-five aflp primer combinations produced 532 aflp markers fitting for map strategy in mapping family. the markers with 3:1 segregating ratios were analyzed using f2 intercross model for the common linkage map, while the markers with 1:1 ratio were analyzed using the pseudo-testcross strategy. the maps of male, female and common were constructed. the female map included 103 markers that formed 28 linkage groups, covering a total length of 1090 m all markers were linked with the linkage groups. segregation distortion was observed for 6 of 103 markers in the female map. the average distance between markers was 14.53 cm and ranged from 4.4 to 24.8 m the male map included 144 markers that formed 35 linkage groups. ten markers remained unlinked in male map. segregation distortion was observed for 7 of 144 markers in the male map. the total distance of male map covered 1617 m the average distance between markers was 16.36 cm. the male map was 32.6% longer than the female map, which may reflect sex-specific recombination rates in chinese shrimp. the common map was composed of 216 markers, including in 44 linkage groups covering a total distance of 1772.1 cm. two markers remained unlinked. no distorted markers of 216 markers were shown in the common map. the distance between markers was 10.42 m an average estimated genome size for the chinese shrimp was 2420 cm, which was consistent with the relative size of the penaeid genome. the distribution of aflp markers was relatively even in chromosomes of chinese shrimp maps. the linkage analysis presented in this work provided some insight into the level of polymorphism and genetic variation of chinese shrimp.
WOS关键词PACIFIC WHITE SHRIMP ; PENAEUS LITOPENAEUS VANNAMEI ; TROUT ONCORHYNCHUS-MYKISS ; QUANTITATIVE TRAIT LOCI ; FENNEROPENAEUS-CHINENSIS ; KURUMA PRAWN ; ICTALURUS-PUNCTATUS ; RECOMBINATION RATES ; MOLECULAR MARKERS ; CHANNEL CATFISH
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
语种英语
WOS记录号WOS:000254999100014
出版者SCIENCE CHINA PRESS
URI标识http://www.irgrid.ac.cn/handle/1471x/2393463
专题中国科学院大学
通讯作者Kong Jie
作者单位1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Dalian Fisheries Univ, Coll Life Sci & Technol, Dalian 116023, Peoples R China
3.Chinese Acad Fisheries Sci, Inst Oceanol, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Tian Yi,Kong Jie,Wang WeiJi. Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis[J]. Chinese science bulletin,2008,53(8):1205-1216.
APA Tian Yi,Kong Jie,&Wang WeiJi.(2008).Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis.Chinese science bulletin,53(8),1205-1216.
MLA Tian Yi,et al."Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis".Chinese science bulletin 53.8(2008):1205-1216.

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来源:中国科学院大学

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