Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis
文献类型:期刊论文
作者 | Tian Yi1,2,3,4; Kong Jie1; Wang WeiJi1 |
刊名 | Chinese science bulletin
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出版日期 | 2008-04-01 |
卷号 | 53期号:8页码:1205-1216 |
关键词 | Aflp Fenneropaeneus chinensis Genetic linkage maps |
ISSN号 | 1001-6538 |
DOI | 10.1007/s11434-008-0170-3 |
通讯作者 | Kong jie() |
英文摘要 | Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in china. a genetic linkage map is essential for improving the efficiency of its breeding by marker-assisted selection and identifying commercially important genes. linkage maps of f chinensis were constructed with an f2 mapping population (110 progenies) using amplified fragment length polymorphic (aflp) marker in this study. fifty-five aflp primer combinations produced 532 aflp markers fitting for map strategy in mapping family. the markers with 3:1 segregating ratios were analyzed using f2 intercross model for the common linkage map, while the markers with 1:1 ratio were analyzed using the pseudo-testcross strategy. the maps of male, female and common were constructed. the female map included 103 markers that formed 28 linkage groups, covering a total length of 1090 m all markers were linked with the linkage groups. segregation distortion was observed for 6 of 103 markers in the female map. the average distance between markers was 14.53 cm and ranged from 4.4 to 24.8 m the male map included 144 markers that formed 35 linkage groups. ten markers remained unlinked in male map. segregation distortion was observed for 7 of 144 markers in the male map. the total distance of male map covered 1617 m the average distance between markers was 16.36 cm. the male map was 32.6% longer than the female map, which may reflect sex-specific recombination rates in chinese shrimp. the common map was composed of 216 markers, including in 44 linkage groups covering a total distance of 1772.1 cm. two markers remained unlinked. no distorted markers of 216 markers were shown in the common map. the distance between markers was 10.42 m an average estimated genome size for the chinese shrimp was 2420 cm, which was consistent with the relative size of the penaeid genome. the distribution of aflp markers was relatively even in chromosomes of chinese shrimp maps. the linkage analysis presented in this work provided some insight into the level of polymorphism and genetic variation of chinese shrimp. |
WOS关键词 | PACIFIC WHITE SHRIMP ; PENAEUS LITOPENAEUS VANNAMEI ; TROUT ONCORHYNCHUS-MYKISS ; QUANTITATIVE TRAIT LOCI ; FENNEROPENAEUS-CHINENSIS ; KURUMA PRAWN ; ICTALURUS-PUNCTATUS ; RECOMBINATION RATES ; MOLECULAR MARKERS ; CHANNEL CATFISH |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
语种 | 英语 |
WOS记录号 | WOS:000254999100014 |
出版者 | SCIENCE CHINA PRESS |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393463 |
专题 | 中国科学院大学 |
通讯作者 | Kong Jie |
作者单位 | 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Dalian Fisheries Univ, Coll Life Sci & Technol, Dalian 116023, Peoples R China 3.Chinese Acad Fisheries Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Tian Yi,Kong Jie,Wang WeiJi. Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis[J]. Chinese science bulletin,2008,53(8):1205-1216. |
APA | Tian Yi,Kong Jie,&Wang WeiJi.(2008).Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis.Chinese science bulletin,53(8),1205-1216. |
MLA | Tian Yi,et al."Construction of aflp-based genetic linkage maps for the chinese shrimp fenneropaeneus chinensis".Chinese science bulletin 53.8(2008):1205-1216. |
入库方式: iSwitch采集
来源:中国科学院大学
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