Derivation and transcriptional profiling analysis of pluripotent stem cell lines from rat blastocysts
文献类型：期刊论文
| 作者 | Li, Chunliang1,4; Yang, Ying1,4; Gu, Junjie1,2,3; Ma, Yu1,2,3; Jin, Ying1,2,3 |
| 刊名 | Cell research
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 19期号:2页码:173-186 |
| 关键词 | Embryonic stem cells Blastocysts Primitive endoderm Teratomas |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2008.301 |
| 通讯作者 | Jin, ying(yjin@sibs.ac.cn) |
| 英文摘要 | Embryonic stem (es) cells are derived from blastocyst-stage embryos. their unique properties of self-renewal and pluripotency make them an attractive tool for basic research and a potential cell resource for therapy. es cells of mouse and human have been successfully generated and applied in a wide range of research. however, no genuine es cell lines have been obtained from rat to date. in this study, we identified pluripotent cells in early rat embryos using specific antibodies against markers of pluripotent stem cells. subsequently, by modifying the culture medium for rat blastocysts, we derived pluripotent rat es-like cell lines, which expressed pluripotency markers and formed embryoid bodies (ebs) in vitro. importantly, these rat es-like cells were able to produce teratomas. both ebs and teratomas contained tissues from all three embryonic germ layers. in addition, from the rat es-like cells, we derived a rat primitive endoderm (pre) cell line. furthermore, we conducted transcriptional profiling of the rat es-like cells and identified the unique molecular signature of the rat pluripotent stem cells. our analysis demonstrates that multiple signaling pathways, including the bmp, activin and mtor pathways, may be involved in keeping the rat es-like cells in an undifferentiated state. the cell lines and information obtained in this study will accelerate our understanding of the molecular regulation underlying pluripotency and guide us in the appropriate manipulation of es cells from a particular species. |
| WOS关键词 | SELF-RENEWAL ; EFFICIENT DERIVATION ; PRIMITIVE ENDODERM ; FACTOR OCT-4 ; MOUSE ; ESTABLISHMENT ; DIFFERENTIATION ; CULTURE ; GROWTH ; FGF |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263287500004 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393593 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jin, Ying |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Chinese Acad Sci,Key Lab Stem Cell Biol, Shanghai Inst Biol Sci,Inst Hlth Sci, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai Stem Cell Inst, Shanghai 200025, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chunliang,Yang, Ying,Gu, Junjie,et al. Derivation and transcriptional profiling analysis of pluripotent stem cell lines from rat blastocysts[J]. Cell research,2009,19(2):173-186. |
| APA | Li, Chunliang,Yang, Ying,Gu, Junjie,Ma, Yu,&Jin, Ying.(2009).Derivation and transcriptional profiling analysis of pluripotent stem cell lines from rat blastocysts.Cell research,19(2),173-186. |
| MLA | Li, Chunliang,et al."Derivation and transcriptional profiling analysis of pluripotent stem cell lines from rat blastocysts".Cell research 19.2(2009):173-186. |
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来源：中国科学院大学
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