The mouse pol i terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like rnas with precise 3' ends in a plasmid-only-based virus rescue system
文献类型：期刊论文
| 作者 | Feng, Liqiang1,2; Li, Feng1; Zheng, Xuehua1; Pan, Weiqi1; Zhou, Kai2,3; Liu, Yichu1; He, Hongxuan3; Chen, Ling1 |
| 刊名 | Archives of virology
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 154期号:7页码:1151-1156 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-009-0422-9 |
| 通讯作者 | Li, feng(li_feng@gibh.ac.cn) |
| 英文摘要 | Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral rna: one uses the self-cleaving hepatitis delta virus ribozyme (hdvr), and the other uses the murine rna polymerase i (pol i) terminator. in this study, we employed egfp and renilla luciferase reporter constructs to compare the efficiency of both methods. our results indicate that the murine pol i terminator was more efficient than the hdvr, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other rna virus rescue systems. |
| WOS关键词 | REVERSE GENETICS SYSTEM ; A VIRUS ; PACKAGING SIGNALS ; SEGMENTS ; VIRIONS ; REGIONS ; GENES ; VRNA ; DNA |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000267680000016 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393872 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Feng |
| 作者单位 | 1.Chinese Acad Sci, Ctr Vaccines & Biotherapeut, Guangzhou Inst Biomed & Hlth, Guangzhou 510663, Guangdong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Natl Res Ctr Wildlife Born Dis, Inst Zool, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Liqiang,Li, Feng,Zheng, Xuehua,et al. The mouse pol i terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like rnas with precise 3' ends in a plasmid-only-based virus rescue system[J]. Archives of virology,2009,154(7):1151-1156. |
| APA | Feng, Liqiang.,Li, Feng.,Zheng, Xuehua.,Pan, Weiqi.,Zhou, Kai.,...&Chen, Ling.(2009).The mouse pol i terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like rnas with precise 3' ends in a plasmid-only-based virus rescue system.Archives of virology,154(7),1151-1156. |
| MLA | Feng, Liqiang,et al."The mouse pol i terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like rnas with precise 3' ends in a plasmid-only-based virus rescue system".Archives of virology 154.7(2009):1151-1156. |
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来源：中国科学院大学
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