Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing
文献类型：期刊论文
| 作者 | Hu, Kejia1,3; Gao, Yunhua1; Zhou, Wenwen1,3; Lian, Jie1,3; Li, Fang1; Chen, Zhifeng2 |
| 刊名 | Langmuir
 |
| 出版日期 | 2009-11-03 |
| 卷号 | 25期号:21页码:12404-12407 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/la901588x |
| 通讯作者 | Gao, yunhua(yhgao@mail.ipc.ac.cn) |
| 英文摘要 | This paper describes a simple method for preparing protein microarrays that is compatible with high throughput manufacturing, the microarrays were formed by maskless photolithography and pin spotting to study protein adsorption on a fluorinated/methoxy-poly(ethylene glycol) (peg) self-assembled monolayer (sam). the mixed nonionic surfactants, tween20 and peg200, were utilized to control nonspecific protein adsorption oil both of the sams. measurements using double-antibody sandwich quantum dots-linked immunosorbent assay (das-qdlisa) showed that the fluorinated sam could effectively minimize nonspecific adsorption in the presence of tween 20 and peg 200 (inhibitors of nonspecific protein adsorption (inspas)) while the pegylated surface was biofouling. additionally, pin spotting was used to fabricate high-throughput protein arrays oil the fluorinated sam. the results displayed that fluorinated sam could not only effectively immobilize protein by hydrophobic interactions, but could also resist the other nonspecific adsorption with the inspa. in this way, protein microarrays would be formed more conveniently and environmentally friendly. it is believed that simple, practical, and high-throughput protein immunosensing could be established with these mixed nonionic surfactants. |
| WOS关键词 | SELF-ASSEMBLED MONOLAYERS ; POLY(ETHYLENE GLYCOL) ; CHAIN DENSITY ; ADHESION ; GOLD |
| WOS研究方向 | Chemistry ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000271106600002 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393892 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, Yunhua |
| 作者单位 | 1.Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China 2.Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Kejia,Gao, Yunhua,Zhou, Wenwen,et al. Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing[J]. Langmuir,2009,25(21):12404-12407. |
| APA | Hu, Kejia,Gao, Yunhua,Zhou, Wenwen,Lian, Jie,Li, Fang,&Chen, Zhifeng.(2009).Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing.Langmuir,25(21),12404-12407. |
| MLA | Hu, Kejia,et al."Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing".Langmuir 25.21(2009):12404-12407. |
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来源：中国科学院大学
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