A system for screening agonists targeting beta(2)-adrenoceptor from chinese medicinal herbs
文献类型：期刊论文
| 作者 | Wang, Hui1,2; Li, Shi-you1; Zhao, Chuan-ke1,2; Zeng, Xin1,2 |
| 刊名 | Journal of zhejiang university-science b
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 10期号:4页码:243-250 |
| ISSN号 | 1673-1581 |
| 关键词 | Beta(2)-adrenoceptor Enhanced green fluorescent protein (egfp) Chinese medicinal herbs Screening |
| DOI | 10.1631/jzus.b0820340 |
| 通讯作者 | Li, shi-you(lishiyou@big.ac.cn) |
| 英文摘要 | In order to develop a model for screening the agonists of human beta(2)-adrenoceptor from chinese medicinal herbs extracts, we used a cell-based functional assay based on a common g protein-coupled receptor (gpcr) regulation mechanism and destabilized enhanced green fluorescent protein (d(2)egfp) reporter gene technique. the positive cell clone was confirmed by real-time polymerase chain reaction (pcr) and imaging analysis. to assess the value of this model, we screened over 2000 high performance liquid chromatography (hplc)-fractionated samples from the ethanol extracts of chinese medicinal herbs. six fractions (isolated from panax japonicus, veratrum nigrum, phellodendron amurense, fructus aurantii immaturus, chaenomeles speciosa, and dictamnus dasycarpus) showed significant effects on active reporter gene expression, three of which (isolated from phellodendron amurense, fructus aurantii immaturus, and chaenomeles speciosa) were selected for further concentration response analysis and the half maximal effective concentration (ec1/2 max) values were 4.2, 2.7, and 4.8 mu g/ml, respectively. therefore, this reporter gene assay was suitable for screening beta(2)-adrenoceptor agonists. the results suggest that the six herbal extracts are the possible agonists of beta(2)-adrenoceptor. |
| WOS关键词 | FLUORESCENT PROTEIN GFP ; GENE ; RECEPTOR ; INHIBITION ; MECHANISMS ; EXTRACT ; ASSAYS ; RATS ; CREB |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
| 语种 | 英语 |
| 出版者 | ZHEJIANG UNIV |
| WOS记录号 | WOS:000264623600001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394306 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Shi-you |
| 作者单位 | 1.Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Li, Shi-you,Zhao, Chuan-ke,et al. A system for screening agonists targeting beta(2)-adrenoceptor from chinese medicinal herbs[J]. Journal of zhejiang university-science b,2009,10(4):243-250. |
| APA | Wang, Hui,Li, Shi-you,Zhao, Chuan-ke,&Zeng, Xin.(2009).A system for screening agonists targeting beta(2)-adrenoceptor from chinese medicinal herbs.Journal of zhejiang university-science b,10(4),243-250. |
| MLA | Wang, Hui,et al."A system for screening agonists targeting beta(2)-adrenoceptor from chinese medicinal herbs".Journal of zhejiang university-science b 10.4(2009):243-250. |
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来源：中国科学院大学
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