Isolation of expressed sequences from a specific chromosome of thinopyrum intermedium infected by bydv
文献类型：期刊论文
| 作者 | Jiang, Shu-Mei1; Yin, Wei-Bo1; Hu, Jun1; Shi, Rui1,2; Zhou, Ruo-Nan1,3; Chen, Yu-Hong1; Zhou, Guang-He4; Wang, Richard R. -C.5; Song, Li-Ying1,3; Hu, Zan-Min1 |
| 刊名 | Genome
 |
| 出版日期 | 2009 |
| 卷号 | 52期号:1页码:68-76 |
| 关键词 | Tai-27 Thinopyrum intermedium Chromosome microdissection Hybridization-specific amplification (hsa) Expressed sequences Barley yellow dwarf virus (bydv) |
| ISSN号 | 0831-2796 |
| DOI | 10.1139/g08-108 |
| 通讯作者 | Hu, zan-min(zmhu@genetics.ac.cn) |
| 英文摘要 | To map important ests to specific chromosomes and (or) chromosomal regions is difficult in hexaploid wheat because of its large genome size and serious interference of homoeologous sequences. large-scale est sequencing and subsequent chromosome localization are both laborious and time-consuming. the wheat alien addition line tai-27 contains a pair of chromosomes of thinopyrum intermedium (host) barkworth & d.r. dewey that carry the resistance gene against barley yellow dwarf virus. in this research, we developed a modified technique based on chromosome microdissection and hybridization-specific amplification to isolate expressed sequences from the alien chromosome of tai-27 by hybridization between the dna of the microdissected alien chromosome and cdna of th. intermedium infected by barley yellow dwarf virus. twelve clones were selected, sequenced, and analyzed. three of them were unknown genes without any hit in the genbank database and the other nine were highly homologous with ests of wheat, barley, and (or) other plants in gramineae induced by abiotic or biotic stress. the method used in this research to isolate expressed sequences from a specific chromosome has the following advantages: (i) the obtained expressed sequences are larger in size and have 3' end information and (ii) the operation is less complicated. it would be an efficient improved method for genomics and functional genomics research of polyploid plants, especially for est development and mapping. the obtained expressed sequence data are also informative in understanding the resistance genes on the alien chromosome of tai-27. |
| WOS关键词 | YELLOW-DWARF VIRUS ; MOLECULAR MARKER ; GENOME PROJECT ; ADDITION LINE ; COMMON WHEAT ; RESISTANCE ; GENES ; MICRODISSECTION ; ESTS ; TAGS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000262673100009 |
| 出版者 | NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394360 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Hu, Zan-Min |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.N Carolina State Univ, Forest Biotechnol Grp, Raleigh, NC 27695 USA 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100000, Peoples R China 5.Utah State Univ, USDA ARS, FRRL, Logan, UT 84322 USA |
| 推荐引用方式 GB/T 7714 | Jiang, Shu-Mei,Yin, Wei-Bo,Hu, Jun,et al. Isolation of expressed sequences from a specific chromosome of thinopyrum intermedium infected by bydv[J]. Genome,2009,52(1):68-76. |
| APA | Jiang, Shu-Mei.,Yin, Wei-Bo.,Hu, Jun.,Shi, Rui.,Zhou, Ruo-Nan.,...&Hu, Zan-Min.(2009).Isolation of expressed sequences from a specific chromosome of thinopyrum intermedium infected by bydv.Genome,52(1),68-76. |
| MLA | Jiang, Shu-Mei,et al."Isolation of expressed sequences from a specific chromosome of thinopyrum intermedium infected by bydv".Genome 52.1(2009):68-76. |
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来源：中国科学院大学
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