Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth
文献类型：期刊论文
| 作者 | Ma, Qing-Hu1; Liu, Yun-Chao1,2 |
| 刊名 | Plant cell reports
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 28期号:11页码:1759-1765 |
| 关键词 | Isopentenyl transferase gene (ipt) Leaf senescence Root growth Transgenic tobacco |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-009-0776-1 |
| 通讯作者 | Ma, qing-hu(mqh@ibcas.ac.cn) |
| 英文摘要 | A cytokinin biosynthetic gene encoding isopentenyl transferase (ipt) was cloned with its native promoter from agrobacterium tumefaciens and introduced into tobacco plants. indolebutyric acid was applied in rooting medium and morphologically normal transgenic tobacco plants were regenerated. genetic analysis of self-fertilized progeny showed that a single copy of intact ipt gene had been integrated, and t(2) progeny had become homozygous for the transgene. stable inheritance of the intact ipt gene in t(2) progeny was verified by southern hybridization. northern blot hybridization revealed that the expression of this ipt gene was confined in leaves and stems but undetectable in roots of the transgenic plants. endogenous cytokinin levels in the leaves and stems of the transgenic tobaccos were two to threefold higher than that of control, but in roots, both the transgenic and control tobaccos had similar cytokinin levels. the elevated cytokinin levels in the transgenic tobacco leaves resulted in delayed leaf senescence in terms of chlorophyll content without affecting the net photosynthetic rate. the root growth and morphology of the plant were not affected in the transgenic tobacco. |
| WOS关键词 | TRANSFORMED PLANT-CELLS ; TRANSGENIC TOBACCO ; CYTOKININ BIOSYNTHESIS ; PROMOTER ; ARABIDOPSIS ; SEQUENCE ; AUXIN ; DNA ; METABOLISM ; SHOOT |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000271905900015 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394367 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma, Qing-Hu |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Qing-Hu,Liu, Yun-Chao. Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth[J]. Plant cell reports,2009,28(11):1759-1765. |
| APA | Ma, Qing-Hu,&Liu, Yun-Chao.(2009).Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth.Plant cell reports,28(11),1759-1765. |
| MLA | Ma, Qing-Hu,et al."Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth".Plant cell reports 28.11(2009):1759-1765. |
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来源：中国科学院大学
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