The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves
文献类型：期刊论文
| 作者 | Xu, Jin1; Chai, Tuanyao1; Zhang, Yuxiu2; Lang, Minglin1; Han, Lu1 |
| 刊名 | Plant cell reports
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 28期号:8页码:1235-1242 |
| 关键词 | Heavy metal Cation-efflux transporter Brassica juncea l. Transgenic plant |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-009-0723-1 |
| 通讯作者 | Chai, tuanyao(tychai@gucas.ac.cn) |
| 英文摘要 | Brassica juncea l. is a zn/cd accumulator. to determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter bjcet2 were investigated using transgenic technology. heterologous expression of bjcet2 in the double mutant yeast strain delta zrc1 delta cot1 enhanced the metal tolerance of the yeast strain and led to decrease in zn or cd accumulation. detection of green fluorescence from green fluorescent protein (gfp) in the root tip of transgenic tobacco further revealed that bjcet2::gfp is localized at the plasma membrane. semi-quantitative rt-pcr analysis showed that bjcet2 was most abundant in the root and was weakly expressed in the stem and leaves. the expression of bjcet2 was up-regulated by heavy metals. however, exposure to low temperature, salt and drought did not affect the expression of bjcet2. overexpression of bjcet2 in transgenic b. juncea plants conferred heavy metal tolerance and increased cd/zn accumulation in the leaves. bjcet2-deficient b. juncea mediated by antisense rna resulted in hypersensitivity to heavy metals and decreased zn/cd accumulation in the plants. these results suggest that the heavy metal efflux of bjcet2 plays important roles in the metal tolerance of b. juncea and in zn/cd accumulation in b. juncea. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; MEMBRANE ; TOBACCO ; ZN |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000268500400008 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394389 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Tuanyao |
| 作者单位 | 1.Chinese Acad Sci, Coll Life Sci, Grad Univ, Beijing 100049, Peoples R China 2.China Univ Min & Technol Beijing, Dept Bioengn, Sch Chem & Environm Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jin,Chai, Tuanyao,Zhang, Yuxiu,et al. The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves[J]. Plant cell reports,2009,28(8):1235-1242. |
| APA | Xu, Jin,Chai, Tuanyao,Zhang, Yuxiu,Lang, Minglin,&Han, Lu.(2009).The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves.Plant cell reports,28(8),1235-1242. |
| MLA | Xu, Jin,et al."The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves".Plant cell reports 28.8(2009):1235-1242. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。