Improving the thermostability of n-carbamyl-d-amino acid amidohydrolase by error-prone pcr
文献类型：期刊论文
| 作者 | Yu, Hong1,2; Li, Jian1,2; Zhang, Dalong1,2; Yang, Yunliu1; Jiang, Weihong1; Yang, Sheng1,3,4 |
| 刊名 | Applied microbiology and biotechnology
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 82期号:2页码:279-285 |
| 关键词 | N-carbamyl-d-amino acid amidohydrolase Thermostability D-amino acid Error-prone pcr |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-008-1748-z |
| 通讯作者 | Yang, sheng(syang@sibs.ac.cn) |
| 英文摘要 | To facilitate the easier production of d-amino acids using n-carbamyl-d-amino acid amidohydrolase (dcase) in an immobilized form, we improved the enzymatic thermostability of highly soluble dcase-m3 of ralstonia pickettii using directed mutagenesis. six novel mutation sites were identified in this study, apart from several thermostability-related amino acid sites reported previously. the most thermostable mutant, in which the 12th amino acid had been changed from glutamine to leucine, showed a 7 a degrees c increase in thermostability. comparative characterization of the parental and mutant dcases showed that although there was a slight reduction in the oxidative stability of the mutants, their kinetic properties and high solubility were not affected. the mutated enzymes are expected to be applied to the development of a fully enzymatic process for the industrial production of d-amino acids. |
| WOS关键词 | SITE-DIRECTED MUTAGENESIS ; CRYSTAL-STRUCTURE ; PROTEIN THERMOSTABILITY ; THERMAL-STABILITY ; SUBSTITUTIONS ; DETERMINANTS ; EVOLUTION ; BURKHOLDERIA ; IMPROVEMENT ; PICKETTII |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263058000010 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394414 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Sheng |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Lab Synthet Biol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Huzhou Res Ctr Ind Biotechnolgy, Huzhou 313000, Peoples R China 4.Shanghai Res & Dev Ctr Ind Biotechnol, Shanghai 201201, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Hong,Li, Jian,Zhang, Dalong,et al. Improving the thermostability of n-carbamyl-d-amino acid amidohydrolase by error-prone pcr[J]. Applied microbiology and biotechnology,2009,82(2):279-285. |
| APA | Yu, Hong,Li, Jian,Zhang, Dalong,Yang, Yunliu,Jiang, Weihong,&Yang, Sheng.(2009).Improving the thermostability of n-carbamyl-d-amino acid amidohydrolase by error-prone pcr.Applied microbiology and biotechnology,82(2),279-285. |
| MLA | Yu, Hong,et al."Improving the thermostability of n-carbamyl-d-amino acid amidohydrolase by error-prone pcr".Applied microbiology and biotechnology 82.2(2009):279-285. |
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来源：中国科学院大学
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