Ipt gene transformation in petunia by an agrobacterium mediated method
文献类型：期刊论文
| 作者 | Bai, L. J.1,2; Ye, C. J.1; Lu, J. Y.1; Yang, D. E.1; Xue, H.1; Pan, Y.1; Cao, P. X.1; Wang, B.1; Liu, M.1 |
| 刊名 | Journal of immunoassay & immunochemistry
 |
| 出版日期 | 2009 |
| 卷号 | 30期号:2页码:224-231 |
| 关键词 | Genetic transformation Isopentenyl transferase gene (ipt) Petunia Race Senescence Tail-pcr |
| ISSN号 | 1532-1819 |
| DOI | 10.1080/15321810902782913 |
| 通讯作者 | Wang, b.(bwang@genetics.ac.cn) |
| 英文摘要 | To prevent leaf senescence of petunia, the cytokinin biosynthetic gene isopentenyl transferase (ipt) was placed under the control of 35s promoter and introduced into petunia. pcr analysis showed an expected 0.5 kb fragment of ipt gene in transgenic petunia. rt-pcr analysis indicated the expression of ipt gene in the transgenic lines. leaves from transgenic plants remained green and healthy in normal culture condition, while the non-transformed plants turned to yellow. transgenic plants showed a reduction in height and smaller leaf sizes. in transgenic lines, the internodes were shorter, and the roots grew slower than the non-transformed plants. |
| WOS关键词 | LEAF SENESCENCE ; CYTOKININ |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology ; Medical Laboratory Technology |
| WOS类目 | Biochemical Research Methods ; Immunology ; Medical Laboratory Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000264660100009 |
| 出版者 | TAYLOR & FRANCIS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394560 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, B. |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, L. J.,Ye, C. J.,Lu, J. Y.,et al. Ipt gene transformation in petunia by an agrobacterium mediated method[J]. Journal of immunoassay & immunochemistry,2009,30(2):224-231. |
| APA | Bai, L. J..,Ye, C. J..,Lu, J. Y..,Yang, D. E..,Xue, H..,...&Liu, M..(2009).Ipt gene transformation in petunia by an agrobacterium mediated method.Journal of immunoassay & immunochemistry,30(2),224-231. |
| MLA | Bai, L. J.,et al."Ipt gene transformation in petunia by an agrobacterium mediated method".Journal of immunoassay & immunochemistry 30.2(2009):224-231. |
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来源：中国科学院大学
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