The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16s rdna sequencing analysis
文献类型：期刊论文
| 作者 | Liu, F. H.1,2; Wang, S. B.1; Zhang, J. S.1,2; Zhang, J.1; Yan, X.1; Zhou, H. K.1; Zhao, G. P.1; Zhou, Z. H.1 |
| 刊名 | Journal of applied microbiology
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 106期号:3页码:952-966 |
| 关键词 | 16s rdna Anaerobic digestion Biogas digester Denaturing gradient gel electrophoresis (dgge) Microbial community Pig manure |
| ISSN号 | 1364-5072 |
| DOI | 10.1111/j.1365-2672.2008.04064.x |
| 通讯作者 | Zhou, z. h.(zhouzhihua@sippe.ac.cn) |
| 英文摘要 | To identify the bacterial and archaeal composition in a mesophilic biogas digester treating pig manure and to compare the consistency of two 16s rdna-based methods to investigate the microbial structure. sixty-nine bacterial operational taxonomic units (otu) and 25 archaeal otu were identified by sequencing two 16s rdna clone libraries. most bacterial otu were identified as phyla of firmicutes (47.2% of total clones), bacteroides (35.4%) and spirochaetes (13.2%). methanoculleus bourgensis (29.0%), methanosarcina barkeri (27.4%) and methanospirillum hungatei (10.8%) were the dominant methanogens. only 9% of bacterial and 20% of archaeal otu matched cultured isolates at a similarity index of >= 97%. about 78% of the dominant bacterial (with abundance > 3%) and 83% of archaeal otu were recovered from the denaturing gradient gel electrophoresis (dgge) bands of v3 regions in 16s rdnas. in the digester, most bacterial and archaeal species were uncultured; bacteria belonging to firmicutes, bacteroides and spirochaetes seem to take charge of cellulolysis, proteolysis, acidogenesis, sulfur-reducing and homoacetogenesis; the most methanogens were typical hydrogenotrophic or hydrogenotrophic/aceticlastic; dgge profiles reflected the dominant microbiota. this study gave a first insight of the overall microbial structure in a rural biogas digester and also indicated dgge was useful in displaying its dominant microbiota. |
| WOS关键词 | PROPIONATE-DEGRADING BACTERIUM ; RATE ANAEROBIC BIOREACTOR ; CHAIN FATTY-ACIDS ; MICROBIAL COMMUNITY ; RIBOSOMAL-RNA ; PHYLOGENETIC DIVERSITY ; METHANOGENIC ARCHAEA ; REACTOR PERFORMANCE ; STORAGE PITS ; WASTE-WATER |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263246900025 |
| 出版者 | WILEY-BLACKWELL PUBLISHING, INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394666 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhou, Z. H. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, F. H.,Wang, S. B.,Zhang, J. S.,et al. The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16s rdna sequencing analysis[J]. Journal of applied microbiology,2009,106(3):952-966. |
| APA | Liu, F. H..,Wang, S. B..,Zhang, J. S..,Zhang, J..,Yan, X..,...&Zhou, Z. H..(2009).The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16s rdna sequencing analysis.Journal of applied microbiology,106(3),952-966. |
| MLA | Liu, F. H.,et al."The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16s rdna sequencing analysis".Journal of applied microbiology 106.3(2009):952-966. |
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来源：中国科学院大学
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