High frequency plant regeneration from the cotyledonary node of common bean
文献类型：期刊论文
| 作者 | Dang, W.1,2; Wei, Z. M.1 |
| 刊名 | Biologia plantarum
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 53期号:2页码:312-316 |
| 关键词 | Bud induction Phaseolus vulgaris Shoot development Silver nitrate Thidiazuron |
| ISSN号 | 0006-3134 |
| DOI | 10.1007/s10535-009-0056-5 |
| 通讯作者 | Wei, z. m.(zmwei@sippe.ac.cn) |
| 英文摘要 | An efficient regeneration system for phaseolus vulgaris was developed from mature seeds germinated on murashige and skoog (ms) medium supplemented with thidiazuron or n(6)-benzylaminopurine (ba) for 6 d. using cotyledonary nodes, multiple buds were induced on the ms medium supplemented with 5.0 mg dm(-3) ba with the induction frequency 71.9 % after 4-week culture. the buds were then transferred onto shoot formation medium containing 1.0 mg dm(-3) ba, 0.1 mg dm(-3) gibberellic acid and 2.0 mg dm(-3) silver nitrate. the addition of agno(3) enhanced the frequency of the shoot formation from 61.3 to 87.6 %. root induction medium was half-strength ms medium with 0.75 mg dm(-3) indolebutyric acid and 0.02 mg dm(-3) ba. the average root frequency was 84.3 %. the regenerated plantlets with healthy roots grew successfully when transferred to soil. using this system we obtained over 10 regenerated plantlets from one explant. |
| WOS关键词 | PHASEOLUS-VULGARIS L ; SHOOT FORMATION ; INTACT SEEDLINGS ; SILVER-NITRATE ; CULTURES ; ORGANOGENESIS ; THIDIAZURON ; ACUTIFOLIUS ; MERISTEMS ; EXPLANTS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000267680100016 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2394803 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wei, Z. M. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dang, W.,Wei, Z. M.. High frequency plant regeneration from the cotyledonary node of common bean[J]. Biologia plantarum,2009,53(2):312-316. |
| APA | Dang, W.,&Wei, Z. M..(2009).High frequency plant regeneration from the cotyledonary node of common bean.Biologia plantarum,53(2),312-316. |
| MLA | Dang, W.,et al."High frequency plant regeneration from the cotyledonary node of common bean".Biologia plantarum 53.2(2009):312-316. |
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来源：中国科学院大学
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