Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm
文献类型：期刊论文
| 作者 | Du, Tanxin1,2; Liu, Bailing1; Hou, Xiaohui1,2; Zhang, Baotan1,2; Du, Cuiming1,2 |
| 刊名 | Applied surface science
 |
| 出版日期 | 2009-06-30 |
| 卷号 | 255期号:18页码:7937-7941 |
| 关键词 | Covalent immobilization Bsa-spacer Conformational change Monodisperse microspheres |
| ISSN号 | 0169-4332 |
| DOI | 10.1016/j.apsusc.2009.04.172 |
| 通讯作者 | Liu, bailing(blliuchem@hotmail.com) |
| 英文摘要 | In this study, the immobilization of glucose oxidase (god) onto micron-size monodisperse poly(styrene-glycidyl methacrylate-sodium sulfonate styrene) microspheres was investigated. in order to improve their surface biocompatibility, bovine serum albumin (bsa) was introduced onto the microspheres' surface as a new type of spacer arm. both the immobilization amount and enzymatic activity were determined in different experimental conditions (enzyme concentration, ph, temperature, etc.). it was found that bsa could serve as a good spacer because it increases both the immobilization amount and enzymatic activity of god. the tolerance of immobilized god against ph, temperatures was examined. moreover, the kinetic parameters for native and immobilized god were obtained and compared. (c) 2009 elsevier b. v. all rights reserved. |
| WOS关键词 | DISPERSION POLYMERIZATION ; TRYPSIN ; MODEL ; BEADS |
| WOS研究方向 | Chemistry ; Materials Science ; Physics |
| WOS类目 | Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| WOS记录号 | WOS:000267469500015 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2395087 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Bailing |
| 作者单位 | 1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Tanxin,Liu, Bailing,Hou, Xiaohui,et al. Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm[J]. Applied surface science,2009,255(18):7937-7941. |
| APA | Du, Tanxin,Liu, Bailing,Hou, Xiaohui,Zhang, Baotan,&Du, Cuiming.(2009).Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm.Applied surface science,255(18),7937-7941. |
| MLA | Du, Tanxin,et al."Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm".Applied surface science 255.18(2009):7937-7941. |
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来源：中国科学院大学
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