Characterization and structural analysis of the coat protein from chinese isolates of cymbidium mosaic virus
文献类型：期刊论文
| 作者 | Liu, Yun-Chao1,3; Li, Lu-Bin2; Li, Yun-Liang1,3; Ma, Qing-Hu1; Peng, Zhen-Hua2 |
| 刊名 | Journal of plant diseases and protection
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 116期号:6页码:241-246 |
| 关键词 | Orchids Phylogenetic reconstruction Plant virus Tetratricopeptide repeat |
| ISSN号 | 1861-3829 |
| 通讯作者 | Ma, qing-hu(mqh@ibcas.ac.cn) |
| 英文摘要 | Cymbidium mosaic virus (cymmv) is the most prevalent virus to infect orchids. the coat protein of cymmv (cycp) is not only necessary for encapsidating the virus rna and cell-to-cell movement, but also a target to create virus-resistant transgenic orchids. in this report, a cymmv coat protein cdna (designated as cycp-cx) was isolated from chinese isolates of cymbidium mosaic virus. an extensive search in genbank database and alignment analysis revealed another 49 cycp isoforms from the orchids in six different countries. these genes plus cycp-cx were used for phylogenetic reconstruction, which revealed three groups, namely group i, ii and iii. the cycp sequences from the same country were generally not clustered together. furthermore, the cycp sequences from the same region or host plant also showed a high divergence. these data indicate that the cymmv has been frequently exchanged among the different countries as a result of the trade of orchids. structural analysis indicates that the cycp is similar to the tetratricopeptide repeat (tpr) protein with predicted three tandem tpr repeats. these tpr domains in cycp structure may have significance in viral disassembly and propagation. |
| WOS关键词 | ODONTOGLOSSUM-RINGSPOT-VIRUS ; TRANSGENIC PLANTS ; GENE ; RESISTANCE ; ORCHIDS ; CLONING ; DESIGN ; RNA |
| WOS研究方向 | Agriculture ; Plant Sciences |
| WOS类目 | Agriculture, Multidisciplinary ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000273454900001 |
| 出版者 | EUGEN ULMER GMBH CO |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2395326 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma, Qing-Hu |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing, Peoples R China 2.Chinese Acad Forestry, Res Inst Forestry, Lab Silviculture State Forestry Adm, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yun-Chao,Li, Lu-Bin,Li, Yun-Liang,et al. Characterization and structural analysis of the coat protein from chinese isolates of cymbidium mosaic virus[J]. Journal of plant diseases and protection,2009,116(6):241-246. |
| APA | Liu, Yun-Chao,Li, Lu-Bin,Li, Yun-Liang,Ma, Qing-Hu,&Peng, Zhen-Hua.(2009).Characterization and structural analysis of the coat protein from chinese isolates of cymbidium mosaic virus.Journal of plant diseases and protection,116(6),241-246. |
| MLA | Liu, Yun-Chao,et al."Characterization and structural analysis of the coat protein from chinese isolates of cymbidium mosaic virus".Journal of plant diseases and protection 116.6(2009):241-246. |
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来源：中国科学院大学
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