Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes
文献类型：期刊论文
| 作者 | Yuan, Ju1,2; Li, Mo1,2; Wei, Liang1,2; Yin, Shen1,2; Xiong, Bo1,2; Li, Sen1,2; Lin, Sheng-Li1,2; Schatten, Heide3; Sun, Qing-Yuan1 |
| 刊名 | Cell cycle
 |
| 出版日期 | 2009-10-15 |
| 卷号 | 8期号:20页码:3384-3395 |
| 关键词 | Astrin Meiotic maturation Spindle assembly Dominant negative Site-directed mutation |
| ISSN号 | 1538-4101 |
| 通讯作者 | Sun, qing-yuan(sunqy1@yahoo.com) |
| 英文摘要 | Astrin has been described as a microtubule and kinetochore protein required for the maintenance of sister chromatid cohesion and centrosome integrity in human mitosis. however, its role in mammalian oocyte meiosis is unclear. in this study, we find that astrin is mainly associated with the meiotic spindle microtubules and concentrated on spindle poles at metaphase i and metaphase ii stages. taxol treatment and immunoprecipitation show that astrin may interact with the centrosomal proteins aurora-a or plk1 to regulate microtubule organization and spindle pole integrity. loss-of-function of astrin by rnai and overexpression of the coiled-coil domain results in spindle disorganization, chromosome misalignment and meiosis progression arrest. thr24, ser66 or ser447 may be the potential phosphorylation sites of astrin by plk1, as site-directed mutation of these sites causes oocyte meiotic arrest at metaphase i with highly disordered spindles and disorganized chromosomes, although mutant astrin localizes to the spindle apparatus. taken together, these data strongly suggest that astrin is critical for meiotic spindle assembly and maturation in mouse oocytes. |
| WOS关键词 | AURORA-A KINASE ; MITOTIC-SPINDLE ; PROTEIN ASTRIN ; CHROMOSOME SEGREGATION ; MICROTUBULE NUCLEATION ; MAMMALIAN OOCYTES ; BOX DOMAIN ; CENTROSOME ; MATURATION ; PLK1 |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000271374300029 |
| 出版者 | LANDES BIOSCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2396080 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Qing-Yuan |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA |
| 推荐引用方式 GB/T 7714 | Yuan, Ju,Li, Mo,Wei, Liang,et al. Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes[J]. Cell cycle,2009,8(20):3384-3395. |
| APA | Yuan, Ju.,Li, Mo.,Wei, Liang.,Yin, Shen.,Xiong, Bo.,...&Sun, Qing-Yuan.(2009).Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes.Cell cycle,8(20),3384-3395. |
| MLA | Yuan, Ju,et al."Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes".Cell cycle 8.20(2009):3384-3395. |
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来源：中国科学院大学
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