Transgenic expression of myb15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in arabidopsis thaliana
文献类型：期刊论文
| 作者 | Ding, Zhenhua1,2; Li, Shiming1,2; An, Xueli1; Liu, Xin1; Qin, Huanju1; Wang, Damen1 |
| 刊名 | Journal of genetics and genomics
 |
| 出版日期 | 2009 |
| 卷号 | 36期号:1页码:17-29 |
| 关键词 | Aba Abiotic stress Arabidopsis thaliana Drought Myb15 Myb transcription factor |
| ISSN号 | 1673-8527 |
| 通讯作者 | Wang, damen(dwwang@genetics.ac.cn) |
| 英文摘要 | Abiotic stresses cause serious crop losses. knowledge on genes functioning in plant responses to adverse growth conditions is essential for developing stress tolerant crops. here we report that transgenic expression of myb15, encoding a r2r3 myb transcription factor in arabidopsis thaliana, conferred hypersensitivity to exogenous abscisic acid (aba) and improved tolerance to drought and salt stresses. the promoter of myb15 was active in not only vegetative and reproductive organs but also the guard cells of stomata. its transcript level was substantially upregulated by aba, drought or salt treatments. compared with wild type (wt) control, myb15 overexpression lines were hypersensitive to aba in germination assays, more susceptible to aba-elicited inhibition of root elongation, and more sensitive to aba-induced stomatal closure. in line with the above findings, the transcript levels of aba biosynthesis (aba1, aba2), signaling (abi3), and responsive genes (atadh1, rd22, rd29b, atem6) were generally higher in myb15 overexpression seedlings than in wt controls after treatment with aba. myb15 overexpression lines displayed improved survival and reduced water loss rates than wt control under water deficiency conditions. these overexpression lines also displayed higher tolerance to nacl stress. collectively, our data suggest that overexpression of myb15 improves drought and salt tolerance in arabidopsis possibly by enhancing the expression levels of the genes involved in aba biosynthesis and signaling, and those encoding the stress-protective proteins. |
| WOS关键词 | ABSCISIC-ACID-INSENSITIVE-3 ABI3 GENE ; MULTIPLE STRESS TOLERANCE ; TRANSCRIPTION FACTOR ; FREEZING TOLERANCE ; SIGNAL-TRANSDUCTION ; RESPONSIVE GENES ; BINDING PROTEIN ; ANION CHANNELS ; PLANT DROUGHT ; SALT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000262749000003 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2396618 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Damen |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Zhenhua,Li, Shiming,An, Xueli,et al. Transgenic expression of myb15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in arabidopsis thaliana[J]. Journal of genetics and genomics,2009,36(1):17-29. |
| APA | Ding, Zhenhua,Li, Shiming,An, Xueli,Liu, Xin,Qin, Huanju,&Wang, Damen.(2009).Transgenic expression of myb15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in arabidopsis thaliana.Journal of genetics and genomics,36(1),17-29. |
| MLA | Ding, Zhenhua,et al."Transgenic expression of myb15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in arabidopsis thaliana".Journal of genetics and genomics 36.1(2009):17-29. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。