Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress
文献类型：期刊论文
| 作者 | Zhang, Li-Fang1,2; Yang, Hao-Meng1; Cui, Su-Xia3; Hu, Jia3; Wang, Jie4; Kuang, Ting-Yun1; Norling, Birgitta5; Huang, Fang1 |
| 刊名 | Journal of proteome research
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 8期号:6页码:2892-2902 |
| 关键词 | Synechocystis sp Strain pcc 6803 High-ph-stress Plasma membrane Subcellular proteomics Two-dimensional electrophoresis Maldi-tof Maldi-tof-tof |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr900024w |
| 通讯作者 | Huang, fang(fhuang@ibcas.ac.cn) |
| 英文摘要 | Cyanobacteria are unique prokaryotes possessing plasma-, outer- and thylakoid membranes. the plasma membrane of a cyanobacterial cell serves as a crucial barrier against its environment and is essential for biogenesis of cyanobacterial photosystems. previously, we have identified 79 different proteins in the plasma membrane of synechocystis sp. strain pcc 6803 based on 213- and 1d- gels and maldi-tof ms. in this work, we have performed a proteomic study screening for high-ph-stress proteins in synechocystis. 2-d gel profiles of plasma membranes isolated from both control and high ph-treated cells were constructed and compared quantitatively based on different protein staining methods including dige analysis. a total of 55 differentially expressed protein spots were identified using malditof ms and maldi-tof/tof ms, corresponding to 39 gene products. twenty-five proteins were enhanced/induced and 14 reduced by high ph. one-third of the enhanced/induced proteins were transport and binding proteins of abc transporters including 3 phosphate transport proteins. other proteins include mind involved in cell division, cya2 in signaling and proteins involved in photosynthesis and respiration. furthermore, among these proteins regulated by high ph, eight were found to be hypothetical proteins. functional significance of the high-ph-stress proteins is discussed integrating current knowledge on cyanobacterial cell physiology. |
| WOS关键词 | CYTOCHROME-C-OXIDASE ; SP PCC-6803 ; ESCHERICHIA-COLI ; SALT-STRESS ; SIGNAL PEPTIDES ; BINDING-PROTEIN ; PHOTOSYSTEM-II ; BICARBONATE TRANSPORT ; PERIPLASMIC PROTEINS ; GEL-ELECTROPHORESIS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000266719400025 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2397273 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Fang |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China 4.Natl Ctr Biomed Anal, Beijing, Peoples R China 5.Stockholm Univ, Dept Biochem & Biophys, Arrhenius Labs Nat Sci, SE-10691 Stockholm, Sweden |
| 推荐引用方式 GB/T 7714 | Zhang, Li-Fang,Yang, Hao-Meng,Cui, Su-Xia,et al. Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress[J]. Journal of proteome research,2009,8(6):2892-2902. |
| APA | Zhang, Li-Fang.,Yang, Hao-Meng.,Cui, Su-Xia.,Hu, Jia.,Wang, Jie.,...&Huang, Fang.(2009).Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress.Journal of proteome research,8(6),2892-2902. |
| MLA | Zhang, Li-Fang,et al."Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress".Journal of proteome research 8.6(2009):2892-2902. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。