Expression and regulation of dickkopf2 during periimplantation in mice
文献类型：期刊论文
| 作者 | Zhang, Ying1,2; Peng, Sha1,2; Kuang, Haibin1,2; Chen, Qi1,2; Liu, Shuang1,2; Zhang, Li1,2; Duan, Enkui1 |
| 刊名 | Journal of reproduction and development
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 55期号:1页码:17-22 |
| 关键词 | Decidualization Dickkopf2 (dkk2) Implantation Mouse Uterus |
| ISSN号 | 0916-8818 |
| 通讯作者 | Duan, enkui(duane@ioz.ac.cn) |
| 英文摘要 | Successful implantation depends oil active dialogue between the maternal endometrium and the implanting blastocysts that is well controlled by groups of regulators at the molecular level, dickkopf2 (dkk2) is a member of dickkopf family normally acting as an antagonist of canonical wnt/beta-catenin signaling, which has been proven to participate in tumorigenesis and early embryo development. in order to explore the potential function of dkk2 in embryo implantation, the present study investigated the uterine expression and regulation profiles of dkk2 during periimplantation in mice. using reverse transcription-polymerase chain reaction, immunohistochemistry and western blotting, we showed that the mrna and protein levels of dkk2 began to increase in the glandular epithelium on day 4, continued to increase on day 5 and then decreased from day 6 of pregnancy. moreover, on days 5-8 of pregnancy, dkk2 was increasingly expressed in the deciduum of file uterus, especially around the implanting embryos. in addition upregulation of dkk2 was also observed in uteri treated with estrogen (estradiol-17 beta) as well as in oil-induced artificial decidualization, indicating that the expression of dkk2 could be induced by both steroid hormone (estrogen) and the process of decidualization. furthermore, in the postimplantation uterus, the dkk2 protein showed all inversed expression with active beta-catenin from day 6 onward, supporting the notion that dkk2 plays an inhibitory role against canonical writ signaling in the context of the decidualizing stroma. collectively, our data suggests that dkk2 expression is associated with uterine receptivity changes its well as the process of decidualization and that it might play important roles through inhibition of canonical writ signaling in the periimplantation uterus. |
| WOS关键词 | LEUKEMIA INHIBITORY FACTOR ; SIGNALING PATHWAY ; GROWTH-FACTORS ; IMPLANTATION ; WNT ; MOUSE ; UTERUS ; BLASTOCYST ; EPITHELIUM ; GENE |
| WOS研究方向 | Agriculture ; Reproductive Biology |
| WOS类目 | Agriculture, Dairy & Animal Science ; Reproductive Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263987700003 |
| 出版者 | SOCIETY REPRODUCTION & DEVELOPMENT-SRD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2397294 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Duan, Enkui |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ying,Peng, Sha,Kuang, Haibin,et al. Expression and regulation of dickkopf2 during periimplantation in mice[J]. Journal of reproduction and development,2009,55(1):17-22. |
| APA | Zhang, Ying.,Peng, Sha.,Kuang, Haibin.,Chen, Qi.,Liu, Shuang.,...&Duan, Enkui.(2009).Expression and regulation of dickkopf2 during periimplantation in mice.Journal of reproduction and development,55(1),17-22. |
| MLA | Zhang, Ying,et al."Expression and regulation of dickkopf2 during periimplantation in mice".Journal of reproduction and development 55.1(2009):17-22. |
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来源：中国科学院大学
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