Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic dnazyme
文献类型：期刊论文
| 作者 | Li, Bingling1,2; Du, Yan1,2; Li, Tao1,2; Dong, Shaojun1,2 |
| 刊名 | Analytica chimica acta
 |
| 出版日期 | 2009-10-05 |
| 卷号 | 651期号:2页码:234-240 |
| 关键词 | 3,3 ',5,5 '-tetramethylbenzidine sulfate Dnazyme Colorimetric sensor Hemin |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2009.09.009 |
| 通讯作者 | Dong, shaojun(dongsj@ciac.jl.cn) |
| 英文摘要 | In this work, the suitability of 3,3',5,5'-tetramethylbenzidine sulfate (tmb) as the substrate of a dnazyme catalytic system composed of a guanine-quadruplex dna molecule and hemin was investigated. in the presence of h(2)o(2), the hemin-dna complex catalyzes the oxidation of tmb to produce two colored products, much like a peroxidase. the color-generating activity of this system could be influenced by several factors such as buffer type, ph value, dna sequence, reaction time, and concentrations of both the hemin and h(2)o(2). to illustrate the utility of this catalytic system, we designed a colorimetric assay, in which a synthetic oligonucleotide with a sequence complementary to the g-quadruplex dna was used as the target. a detection limit of 1.86 nm was obtained. our data have shown that tmb was an excellent colorimetric indicator that reported the peoxidase activities of the widely studied hemin-g-quadruplex dnazyme system. (c) 2009 elsevier b.v. all rights reserved. |
| WOS关键词 | CATALYTIC DNA ; CLEAVING DNA ; COMPLEX ; ENZYME ; LABEL ; THROMBIN ; ASSAY |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000270745600015 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2397490 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dong, Shaojun |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Bingling,Du, Yan,Li, Tao,et al. Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic dnazyme[J]. Analytica chimica acta,2009,651(2):234-240. |
| APA | Li, Bingling,Du, Yan,Li, Tao,&Dong, Shaojun.(2009).Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic dnazyme.Analytica chimica acta,651(2),234-240. |
| MLA | Li, Bingling,et al."Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic dnazyme".Analytica chimica acta 651.2(2009):234-240. |
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来源：中国科学院大学
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