Recombinant apoptin gene retrovirus induces apoptosis in human breast cancer cells 435
文献类型：期刊论文
| 作者 | Yu, Chun-mei1; Xu, Hai-tao2; Du, Jie3 |
| 刊名 | Chinese journal of cancer research
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 21期号:3页码:194-201 |
| 关键词 | Apoptin Retrovirus Gene therapy Breast cancer |
| ISSN号 | 1000-9604 |
| DOI | 10.1007/s11670-009-0194-5 |
| 通讯作者 | Yu, chun-mei(chunmeiyu66@163.com) |
| 英文摘要 | To construct recombinant apoptin gene (vp3) retrovirus plvp3 and to study its apoptosis inducing effect on human breast cancer cells 435 as well as to discuss its mechanism in vitro and in vivo. vp3 gene was cloned and recombinated into retrovirus vector plp-lncx-vp3 (plvp3) at loxp site, which was transformed into package cell line pt67 and then into nih3t3 cells for titer assay. the human breast cancer cell line 435 was infected with retrovirus plvp3, and then mtt assay and western blotting were adopted to detect cellular proliferation and apoptin protein expression. forty-eight hours after infection flow cytometry (fcm) was used for apoptosis detection and surface enhanced laser desorption ionization-time of flight-mass spectrometry (seldi-tof-ms) was used for protein profile assay. nude mice model of human breast cancer cells 435 was set up to observe the tumor inhibiting rates of plvp3, and tunel assay was used to detect tumor apoptosis as well as real-time pcr for vp3 gene expression. recombinant plasmid plvp3 was successfully constructed. virus titer reached to 5x10(8) pfu/ml in the pt67 culture supernatant. forty-eight hours after infection, cellular inhibition rate was 65.1% in mtt assay, higher than that in blank control (p < 0.05) and apoptin protein expressed more in test group in western blotting. fcm assay showed apoptotic peaks with a percentage of 15.42%. seldi-tof-ms findings suggested that two protein peaks, m_2544.1+h and m_3712.4+h, were statistically different between infection group and control group (p < 0.05). the tumor inhibition rates in plvp3 group were 65.52% and 68.23%, much higher than that of control group (t=4.06, p < 0.01). tunel assay findings showed that positive yellow stains were seen in plvp3 retrovirus group and 5-fu positive control group without difference (t1=1.05, t2=0.84, p > 0.05). the experiment demonstrated that vp3 could induce apoptosis in tumor cells in vivo and in vitro, which laid a basis for further study on molecular mechanism of tumor cell apoptosis induced by apoptin and provided valuable reference for tumor gene therapy. |
| WOS关键词 | MASS-SPECTROMETRY ; PROSTATE-CANCER ; THEILERS-VIRUS ; PROTEIN ; POLIOVIRUS ; INDUCTION ; REPLICONS ; INFECTION ; RECEPTOR |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000268869500005 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2397714 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Chun-mei |
| 作者单位 | 1.Beijing Chuiyangliu Hosp, Dept Breast Ctr, Beijing 100022, Peoples R China 2.Harbin Med Univ, Affiliated Hosp 3, Dept Abdominal Surg, Harbin 150086, Peoples R China 3.Chinese Acad Sci, Grad Sch, Dept Biol, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Chun-mei,Xu, Hai-tao,Du, Jie. Recombinant apoptin gene retrovirus induces apoptosis in human breast cancer cells 435[J]. Chinese journal of cancer research,2009,21(3):194-201. |
| APA | Yu, Chun-mei,Xu, Hai-tao,&Du, Jie.(2009).Recombinant apoptin gene retrovirus induces apoptosis in human breast cancer cells 435.Chinese journal of cancer research,21(3),194-201. |
| MLA | Yu, Chun-mei,et al."Recombinant apoptin gene retrovirus induces apoptosis in human breast cancer cells 435".Chinese journal of cancer research 21.3(2009):194-201. |
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来源：中国科学院大学
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