Simultaneous degradation of organophosphates and 4-substituted phenols by stenotrophomonas species lz-1 with surface-displayed organophosphorus hydrolase
文献类型：期刊论文
| 作者 | Liu, Zheng2,3; Yang, Chao2,3; Jiang, Hong3; Mulchandani, Ashok1; Chen, Wilfred1; Qiao, Chuanling3 |
| 刊名 | Journal of agricultural and food chemistry
 |
| 出版日期 | 2009-07-22 |
| 卷号 | 57期号:14页码:6171-6177 |
| 关键词 | Simultaneous degradation Organophosphates Oph Ice nucleation protein Surface display 4-substituted phenols |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/jf804008j |
| 通讯作者 | Mulchandani, ashok(adani@engr.ucr.edu) |
| 英文摘要 | Organophosphorous hydrolase (oph) was expressed onto the surface of a stenotrophomonas species (lz-1), capable of simultaneously degrading 4-substituted phenols, using the n- and c-terminal domains of ice nucleation protein (inpnc) as an anchoring motif for the first time. the engineered strain lz-1 could degrade p-nitrophenyl-substituted organophosphates as well as their hydrolytic product, pnp, rapidly. especially, addition of 4-cp (below 0.8 mm) significantly accelerated the complete degradation of above organophosphates (47.1, 34.0, and 40% reduction of time of paraoxon, parathion, and methyl-parathion, respectively) through the accelerated degradation of pnp due to enhanced cell growth supported by 4-cp as the carbon source. oph could be surface-displayed at a high level without inhibition of cell growth and oph activity in the presence of 4-cp. in soil samples, strain lz-1 could also remove these compounds successfully. functional display of heterologous proteins on the surface of indigenous bacteria could provide a promising technology for effective bioremediation of sites contaminated with mixed organic pollutants. |
| WOS关键词 | ICE-NUCLEATION PROTEIN ; INHIBITORY CO-METABOLITE ; PSEUDOMONAS-PUTIDA ; P-NITROPHENOL ; CELL-SURFACE ; BIODEGRADATION KINETICS ; NOCARDIOIDES SP ; NERVE AGENTS ; 4-CHLOROPHENOL ; PARATHION |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000268138100027 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2398316 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Mulchandani, Ashok |
| 作者单位 | 1.Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zheng,Yang, Chao,Jiang, Hong,et al. Simultaneous degradation of organophosphates and 4-substituted phenols by stenotrophomonas species lz-1 with surface-displayed organophosphorus hydrolase[J]. Journal of agricultural and food chemistry,2009,57(14):6171-6177. |
| APA | Liu, Zheng,Yang, Chao,Jiang, Hong,Mulchandani, Ashok,Chen, Wilfred,&Qiao, Chuanling.(2009).Simultaneous degradation of organophosphates and 4-substituted phenols by stenotrophomonas species lz-1 with surface-displayed organophosphorus hydrolase.Journal of agricultural and food chemistry,57(14),6171-6177. |
| MLA | Liu, Zheng,et al."Simultaneous degradation of organophosphates and 4-substituted phenols by stenotrophomonas species lz-1 with surface-displayed organophosphorus hydrolase".Journal of agricultural and food chemistry 57.14(2009):6171-6177. |
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来源：中国科学院大学
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