Molecular analysis of rice plants harboring a multi-functional t-dna tagging system
文献类型：期刊论文
| 作者 | Ma, Yimian1,2,3; Liu, Luo1,2; Zhu, Chengguang1,2; Sun, Changhui1,2; Xu, Bo1,2,3; Fang, Jun1,2; Tang, Jiuyou1,2,3; Luo, Anding1,2; Cao, Shouyun1,2; Li, Gupo1,2 |
| 刊名 | Journal of genetics and genomics
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 36期号:5页码:267-276 |
| 关键词 | Rice Mutant population Promoter trap Activation tag T-dna integration |
| ISSN号 | 1673-8527 |
| DOI | 10.1016/s1673-8527(08)60114-9 |
| 通讯作者 | Chu, chengcai(ccchu@genetics.ac.cn) |
| 英文摘要 | About 25,000 rice t-dna insertional mutant lines were generated using the vector pcas04 which has both promoter-trapping and activation-tagging function. southern blot analysis revealed that about 40% of these mutants were single copy integration and the average t-dna insertion number was 2.28. by extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. histochemical gus assay with 4,3 10 primary mutants revealed that the gus-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. the t-dna flanking sequences of some mutants were isolated and the t-dna insertion sites were mapped to the rice genome. the flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. compared with arabidopsis and poplar, it is much varied in the t-dna border junctions in rice. |
| WOS关键词 | ARABIDOPSIS-THALIANA ; FUNCTIONAL GENOMICS ; ILLEGITIMATE RECOMBINATION ; INSERTIONAL MUTAGENESIS ; TRANSGENE EXPRESSION ; TRAPPING SYSTEM ; DRAFT SEQUENCE ; PROMOTER TRAP ; BINARY VECTOR ; ACTIVATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000266053600001 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2398591 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chu, Chengcai |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Plant Gene Res Ctr Beijing, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 4.Chinese Acad Agr Sci, China Inst Rice Res, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China 5.Chinese Acad Sci, Inst Genet & Dev Biol, Mol & Dev Biol Lab, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Yimian,Liu, Luo,Zhu, Chengguang,et al. Molecular analysis of rice plants harboring a multi-functional t-dna tagging system[J]. Journal of genetics and genomics,2009,36(5):267-276. |
| APA | Ma, Yimian.,Liu, Luo.,Zhu, Chengguang.,Sun, Changhui.,Xu, Bo.,...&Chu, Chengcai.(2009).Molecular analysis of rice plants harboring a multi-functional t-dna tagging system.Journal of genetics and genomics,36(5),267-276. |
| MLA | Ma, Yimian,et al."Molecular analysis of rice plants harboring a multi-functional t-dna tagging system".Journal of genetics and genomics 36.5(2009):267-276. |
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来源：中国科学院大学
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