The heterogeneous nature of polyethylenimine-dna complex formation affects transient gene expression
文献类型：期刊论文
| 作者 | Han, Xiangzong2; Fang, Qiangyi2; Yao, Feng2; Wang, Xiaoning4; Wang, Jufang4; Yang, Shengli2,3; Shen, Bing Q.1,2,3 |
| 刊名 | Cytotechnology
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 60期号:1-3页码:63-75 |
| 关键词 | Cell cycle Flow cytometry Hek 293 Polyethylenimine Particle size Transient gene expression |
| ISSN号 | 0920-9069 |
| DOI | 10.1007/s10616-009-9215-y |
| 通讯作者 | Shen, bing q.(bqshen@nankai.edu.cn) |
| 英文摘要 | Polyethylenimine has been used widely in transient gene expression with mammalian cells. to further understand its mediation of gene transfer, the transfection of hek 293-f cells with dynamically prepared pei/dna complexes was studied with the help of fluorescent labeling. the efficiency of complex endocytosis/phagocytosis was found to correlate with the average sizes of complexes applied and complexes greater than 1 mu m in diameter were likely excluded by the cells. coupled with complex growth in size, the degree of association between pei and dna increased with the time of complex formation in the presence of competing ions. the blocking of transcription by complex formation necessitated complex dissociation in the nuclear environment for transcription to happen. intracellularly, the fates of pei complexed dna therefore may be mostly determined by the degree of association. results also suggested that the uptake of pei/dna complexes and subsequent protein expression were independent of the cell cycle stages of hek 293-f cells. |
| WOS关键词 | FREE SUSPENSION-CULTURE ; MAMMALIAN-CELLS ; TRANSFECTION EFFICIENCY ; PROTEIN-PRODUCTION ; HEK-293 CELLS ; EBNA1 CELLS ; CHO-CELLS ; IN-VIVO ; DELIVERY ; VECTOR |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Cell Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000272027900008 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2398593 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shen, Bing Q. |
| 作者单位 | 1.Nankai Univ, Coll Pharmaceut Sci, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200233, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China 4.S China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Xiangzong,Fang, Qiangyi,Yao, Feng,et al. The heterogeneous nature of polyethylenimine-dna complex formation affects transient gene expression[J]. Cytotechnology,2009,60(1-3):63-75. |
| APA | Han, Xiangzong.,Fang, Qiangyi.,Yao, Feng.,Wang, Xiaoning.,Wang, Jufang.,...&Shen, Bing Q..(2009).The heterogeneous nature of polyethylenimine-dna complex formation affects transient gene expression.Cytotechnology,60(1-3),63-75. |
| MLA | Han, Xiangzong,et al."The heterogeneous nature of polyethylenimine-dna complex formation affects transient gene expression".Cytotechnology 60.1-3(2009):63-75. |
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来源：中国科学院大学
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