Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l.
文献类型：期刊论文
| 作者 | Xu, Gang2,3,4; Li, Chunyang3,4; Yao, Yinan1 |
| 刊名 | Plant molecular biology reporter
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 27期号:2页码:153-161 |
| 关键词 | Drought Two-dimensional gel electrophoresis Hippophae rhamnoides l. Mass spectrometry |
| ISSN号 | 0735-9640 |
| DOI | 10.1007/s11105-008-0067-y |
| 通讯作者 | Yao, yinan(yaoyinan0430@163.com) |
| 英文摘要 | Hippophae rhamnoides l. is uniquely capable of growing well under extreme environmental conditions such as water deficit, low temperature, and high altitude. such tolerance invokes much interest in understanding the biology of this plant species and its utilization potential. in this study, analysis of drought stress-responsive proteins in h. rhamnoides was conducted wherein greenhouse-grown seedlings were subjected to drought stress. by using proteomic techniques, proteins, extracted from leaves, were analyzed using two-dimensional electrophoresis and maldi-tof ms. altogether, 55 proteins exhibited changes in abundance under stress. of these, 13 proteins were identified, including three that disappeared under drought (a putative abc transporter atp-binging protein, a heat shock protein hs1u, and a hypothetical protein xp515578), seven that were up-regulated (three large subunits of rubisco, a hypothetical protein dsm3645-23351, a putative acyl-coa dehydrogenase, a nesprin-2, and a j-type co-chaperone hsc20), and three that were only detected under drought (a probable nitrogen regulation protein (ntrx), a 4-hydroxyphenylpyruvate dioxygenase, and an unnamed protein product). these proteins may function in beta-oxidation pathways in mitochondria, across membranes transport, abnormal protein removal, or prevent protein aggregation arrest, cell division, cytoskeleton stabilization, iron-sulfur cluster assembly, nitrogen metabolism regulation, and antioxidant substance biosynthesis. four proteins (j-type co-chaperone hsc20, a putative abc transporter atp-binging protein, ntrx, and hs1u) were deemed as new discoveries in higher plants, and their functions were predicted either from their conserved domains or homologies to other organisms. these results provide new insights into our understanding of the mechanism of drought tolerance in plants. |
| WOS关键词 | NITRATE REDUCTASE-ACTIVITY ; SYNECHOCYSTIS SP PCC-6803 ; NITROGEN-FIXATION ; ESCHERICHIA-COLI ; PHYSIOLOGICAL-RESPONSES ; ABC TRANSPORTERS ; WATER DEFICITS ; MAIZE LEAVES ; ARABIDOPSIS ; PLANT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemical Research Methods ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000270805900006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2398651 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yao, Yinan |
| 作者单位 | 1.Guizhou Univ, Coll Agr, Guiyang 550025, Peoples R China 2.Guizhou Univ, Key Lab Green Pesticide & Agr Bioengn, Chinese Minist Educ, Ctr Res & Dev Fine Chem, Guiyang 550025, Peoples R China 3.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China 4.Chinese Acad Sci, China Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Gang,Li, Chunyang,Yao, Yinan. Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l.[J]. Plant molecular biology reporter,2009,27(2):153-161. |
| APA | Xu, Gang,Li, Chunyang,&Yao, Yinan.(2009).Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l..Plant molecular biology reporter,27(2),153-161. |
| MLA | Xu, Gang,et al."Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l.".Plant molecular biology reporter 27.2(2009):153-161. |
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来源：中国科学院大学
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