Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting dna sequences
文献类型：期刊论文
| 作者 | Liu Sheng1,2; Chen Miao1; Li JinDu1,2; Zhang HaoBo1,2; Wang JinQing1; Yang ShengRong1 |
| 刊名 | Science in china series b-chemistry
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 52期号:7页码:1009-1013 |
| 关键词 | Azobenzene monomer Peptide nucleic acid Hybridization Gold electrode Electrochemical biosensors |
| ISSN号 | 1006-9291 |
| DOI | 10.1007/s11426-009-0019-x |
| 通讯作者 | Wang jinqing(jqwang@lzb.ac.cn) |
| 英文摘要 | Hybridization of peptide nucleic acids probe containing azobenzene (nh(2)-tnt(4), n-pnas) with dna was performed by covalently immobilizing of nh(2)-tnt(4) in sequence on the 3-mercaptopropionic acid self-assembled monolayer modified gold electrode with the helps of n-(3-dimethylaminopropy1)-n'-ethylcarbodiimide hydrochloride (edc) and n-hydroxysuccinimide (nhs), and the hybrid was coded as n-pnas/dna. using [fe(cn)(6)](4-/3-) (1:1) as the electrochemical indicator, the electrochemical properties of the n-pnas self-assembled monolayer (n-pnas-sams) and n-pnas/dna hybridization system under the conditions of before and after uv light irradiation were characterized with cyclic voltammetry (cv), differential pulse voltammetry (dpv), and electrochemical impedance spectra (eis). results showed that the redox currents decreased with the increase of irradiation time, suggesting that the ability of the charge transfer on the electrode surface was weakened and the conformation of hybrid system had been changed, and the control of pnas/dna hybridization could be realized by uv light irradiation. |
| WOS关键词 | GOLD ELECTRODES ; HYBRIDIZATION ; MONOLAYER |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000267396300022 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2398654 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang JinQing |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Solid Lubricat, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Sheng,Chen Miao,Li JinDu,et al. Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting dna sequences[J]. Science in china series b-chemistry,2009,52(7):1009-1013. |
| APA | Liu Sheng,Chen Miao,Li JinDu,Zhang HaoBo,Wang JinQing,&Yang ShengRong.(2009).Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting dna sequences.Science in china series b-chemistry,52(7),1009-1013. |
| MLA | Liu Sheng,et al."Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting dna sequences".Science in china series b-chemistry 52.7(2009):1009-1013. |
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来源：中国科学院大学
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