Theoretical model and quantification of reflectance photometer
文献类型：期刊论文
| 作者 | Huang, Lihua1,2; Zhang, Youbao1; Xie, Chengke1,2; Qu, Jianfeng1; Huang, Huijie1; Wang, Xiangzhao1 |
| 刊名 | Chinese optics letters
 |
| 出版日期 | 2009-11-10 |
| 卷号 | 7期号:11页码:1031-1034 |
| ISSN号 | 1671-7694 |
| DOI | 10.3788/col20090711.1031 |
| 通讯作者 | Huang, lihua(hlh@siom.ac.cn) |
| 英文摘要 | The surface morphology of lateral flow (lf) strip is examined by scanning electron microscope (sem) and the diffuse reflection of porous strip with or without nanogold particles is investigated. based on the scattering and absorption of nanogold particles, a reflectance photometer is developed for quantification of lf strip with nanogold particles as reporter. the integration of reflection optical density is to indicate the signals of test line and control line. as an example, serial dilutions of microalbunminuria (mau) solution are used to calibrate the performance of the reflectance photometer. the dose response curve is fitted with a four-parameter logistic mathematical model for the determination of an unknown mau concentration. the response curve spans a dynamic range of 5 to 200 mu g/ml. the developed reflectance photometer can realize simple and quantitative detection of analyte on nanogold-labeled lf strip. |
| WOS关键词 | URINARY ALBUMIN EXCRETION ; IMMUNOASSAY |
| WOS研究方向 | Optics |
| WOS类目 | Optics |
| 语种 | 英语 |
| WOS记录号 | WOS:000272346500019 |
| 出版者 | CHINESE OPTICS LETTERS MANUSCRIPT OFFICE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2398912 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Lihua,Zhang, Youbao,Xie, Chengke,et al. Theoretical model and quantification of reflectance photometer[J]. Chinese optics letters,2009,7(11):1031-1034. |
| APA | Huang, Lihua,Zhang, Youbao,Xie, Chengke,Qu, Jianfeng,Huang, Huijie,&Wang, Xiangzhao.(2009).Theoretical model and quantification of reflectance photometer.Chinese optics letters,7(11),1031-1034. |
| MLA | Huang, Lihua,et al."Theoretical model and quantification of reflectance photometer".Chinese optics letters 7.11(2009):1031-1034. |
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来源：中国科学院大学
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