Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization
文献类型：期刊论文
| 作者 | Su, Xiaoyun1,2; Chu, Xin1,2; Dong, Zhiyang1 |
| 刊名 | World journal of microbiology & biotechnology
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 25期号:6页码:1075-1084 |
| 关键词 | Trichoderma reesei Chimeric transcription activator Suppression subtractive hybridization Cre1 Acei Aceii |
| ISSN号 | 0959-3993 |
| DOI | 10.1007/s11274-009-9993-6 |
| 通讯作者 | Dong, zhiyang(dongzy@sun.im.ac.cn) |
| 英文摘要 | To know how the two transcription repressors cre1 and acei affect their downstream genes, a new strategy was employed in which a plasmid encoding a chimeric transcription activator containing the dna binding domains from cre1 and acei and the effector domain from aceii was constructed and transformed into trichoderma reesei. nineteen elevated transcripts were identified in the transformant against its parent strain using suppression subtractive hybridization. all of them had the consensus binding motif for cre1 (5'-syggrg-3') and acei (5'-aggca-3'), among which seven had the most representative binding motif (5'-aggcaaa-3') for acei in their 1,000-bp promoters. cre1 could bind to the promoters of all the genes while acei could bind to the promoters of six out of the seven in vitro. the results provide a primary overview of a set of genes which may be associated to cre1 or acei. it is the first step towards an understanding of the regulatory roles of the two repressors in cellular pathways, which would be helpful in strain improvement in t. reesei. |
| WOS关键词 | CARBON CATABOLITE REPRESSION ; ASPERGILLUS-NIDULANS ; GLUCOSE REPRESSION ; GENE-EXPRESSION ; BINDING-SITES ; CELLULASE ; CLONING ; GROWTH ; TRANSFORMATION ; RECOGNITION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000266168800017 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2399114 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dong, Zhiyang |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Xiaoyun,Chu, Xin,Dong, Zhiyang. Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization[J]. World journal of microbiology & biotechnology,2009,25(6):1075-1084. |
| APA | Su, Xiaoyun,Chu, Xin,&Dong, Zhiyang.(2009).Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization.World journal of microbiology & biotechnology,25(6),1075-1084. |
| MLA | Su, Xiaoyun,et al."Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization".World journal of microbiology & biotechnology 25.6(2009):1075-1084. |
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来源：中国科学院大学
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