Different responsiveness to a high-fat/cholesterol diet in two inbred mice and underlying genetic factors: a whole genome microarray analysis
文献类型：期刊论文
| 作者 | Zhu, Mingzhe1,2,8; Ji, Guozhen3; Jin, Gang1,2,4,5,6; Yuan, Zuobiao1,2,7 |
| 刊名 | Nutrition & metabolism
 |
| 出版日期 | 2009-10-17 |
| 卷号 | 6页码:9 |
| ISSN号 | 1743-7075 |
| DOI | 10.1186/1743-7075-6-43 |
| 通讯作者 | Jin, gang(gjin@sibs.ac.cn) |
| 英文摘要 | Background: to investigate different responses to a high-fat/cholesterol diet and uncover their underlying genetic factors between c57bl/6j (b6) and dba/2j (d2) inbred mice. methods: b6 and d2 mice were fed a high-fat/cholesterol diet for a series of time-points. serum and bile lipid profiles, bile acid yields, hepatic apoptosis, gallstones and atherosclerosis formation were measured. furthermore, a whole genome microarray was performed to screen hepatic genes expression profile. quantitative real-time pcr, western blot and tunel assay were conducted to validate microarray data. results: after fed the high-fat/cholesterol diet, serum and bile total cholesterol, serum cholesterol esters, hdl cholesterol and non-hdl cholesterol levels were altered in b6 but not significantly changed in d2; meanwhile, biliary bile acid was decreased in b6 but increased in d2. at the same time, hepatic apoptosis, gallstones and atherosclerotic lesions occurred in b6 but not in d2. the hepatic microarray analysis revealed distinctly different genes expression patterns between b6 and d2 mice. their functional pathway groups included lipid metabolism, oxidative stress, immune/inflammation response and apoptosis. quantitative real time pcr, tunel assay and western-blot results were consistent with microarray analysis. conclusion: different genes expression patterns between b6 and d2 mice might provide a genetic basis for their distinctive responses to a high-fat/cholesterol diet, and give us an opportunity to identify novel pharmaceutical targets in related diseases in the future. |
| WOS关键词 | CHOLESTEROL GALLSTONE FORMATION ; ATHEROSCLEROSIS ; EXPRESSION ; DISEASE ; 7-ALPHA-HYDROXYLASE ; TRANSPORTERS ; INFLAMMATION ; METABOLISM ; STRAINS ; IMMUNE |
| WOS研究方向 | Nutrition & Dietetics |
| WOS类目 | Nutrition & Dietetics |
| 语种 | 英语 |
| WOS记录号 | WOS:000271632500001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2399265 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jin, Gang |
| 作者单位 | 1.Chinese Acad Sci, Inst Hlth Sci, Shanghai Inst Biol Sci, Shanghai, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200030, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 4.ShanghaiBio Corp, N Brunswick, NJ USA 5.Shanghai Biochip Co Ltd, Shanghai, Peoples R China 6.Natl Engn Ctr Biochip Shanghai, Shanghai, Peoples R China 7.Univ Iowa, Dept Surg, Iowa City, IA 52242 USA 8.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Mingzhe,Ji, Guozhen,Jin, Gang,et al. Different responsiveness to a high-fat/cholesterol diet in two inbred mice and underlying genetic factors: a whole genome microarray analysis[J]. Nutrition & metabolism,2009,6:9. |
| APA | Zhu, Mingzhe,Ji, Guozhen,Jin, Gang,&Yuan, Zuobiao.(2009).Different responsiveness to a high-fat/cholesterol diet in two inbred mice and underlying genetic factors: a whole genome microarray analysis.Nutrition & metabolism,6,9. |
| MLA | Zhu, Mingzhe,et al."Different responsiveness to a high-fat/cholesterol diet in two inbred mice and underlying genetic factors: a whole genome microarray analysis".Nutrition & metabolism 6(2009):9. |
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来源：中国科学院大学
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