The novel epididymis-specific beta-galactosidase-like gene glb1l4 is essential in epididymal development and sperm maturation in rats
文献类型：期刊论文
| 作者 | Zhen, Wei1,2; Li, Peng1; He, Bin1; Guo, Juyuan1; Zhang, Yong-Lian1,3 |
| 刊名 | Biology of reproduction
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 80期号:4页码:696-706 |
| 关键词 | Developmental biology Epididymal development Epididymis Gene regulation Glb1l4 Male reproductive tract Novel gene Principal cell differentiation Sperm Sperm maturation |
| ISSN号 | 0006-3363 |
| DOI | 10.1095/biolreprod.108.071589 |
| 通讯作者 | Zhang, yong-lian(ylzhang@sibs.ac.cn) |
| 英文摘要 | We describe a novel epididymis-specific cdna named glb1l4, which was isolated from rat epididymis by differential display of mrnas. glb1l4 cdna contains 2607 nucleotides and encodes a 637-amino acid protein with 50% similarity to mouse beta-galactosidase. the gene is located on chromosome 8q13, spanning 21 exons. northern blot analysis reveals that glb1l4 is specifically expressed in the caput region of epididymis and upregulated by androgen. a specific polyclonal antiserum against the n-terminal peptide of glb1l4 has been produced. western blot analysis and immunohistochemistry assay reveal that glb1l4 is specifically expressed in the principal cells of the caput epididymis. interestingly, its expression peaks at postnatal day 45 in mrna level and at postnatal day 60 in protein level while the epididymis column cells undergo differentiation. moreover, within this very period this secretory protein is confined inside the cell with a change of subcellular distribution pattern, which implies its important roles in the cell differentiation process. only after the epididymal epithelium differentiation is completed and the spermatozoa enter the epididymal lumen is the glb1l4 secreted into the luminal fluid and bound on the sperm head. our results suggest that glb1l4 may play various roles in principal cell differentiation and sperm maturation. |
| WOS关键词 | LUMINAL FLUID ; PURIFICATION ; EXPRESSION ; PROTEINS ; SYSTEM ; CELLS |
| WOS研究方向 | Reproductive Biology |
| WOS类目 | Reproductive Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000264437000011 |
| 出版者 | SOC STUDY REPRODUCTION |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2400763 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Yong-Lian |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China 3.Shanghai Inst Planned Parenthood Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhen, Wei,Li, Peng,He, Bin,et al. The novel epididymis-specific beta-galactosidase-like gene glb1l4 is essential in epididymal development and sperm maturation in rats[J]. Biology of reproduction,2009,80(4):696-706. |
| APA | Zhen, Wei,Li, Peng,He, Bin,Guo, Juyuan,&Zhang, Yong-Lian.(2009).The novel epididymis-specific beta-galactosidase-like gene glb1l4 is essential in epididymal development and sperm maturation in rats.Biology of reproduction,80(4),696-706. |
| MLA | Zhen, Wei,et al."The novel epididymis-specific beta-galactosidase-like gene glb1l4 is essential in epididymal development and sperm maturation in rats".Biology of reproduction 80.4(2009):696-706. |
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来源：中国科学院大学
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