Expression analysis of rice u-box proteins at different developmental stages
文献类型：期刊论文
| 作者 | Chen Hao1; Li Li-Yun1; Bai Hui2,3; Cao Ying-Hao1; Wang Xian-Yun1; Liu Guo-Zhen1,2 |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 36期号:9页码:1208-1214 |
| 关键词 | Rice U-box Protein expression Western blotting |
| ISSN号 | 1000-3282 |
| DOI | 10.3724/sp.j.1206.2009.00075 |
| 通讯作者 | Liu guo-zhen(gzhliu@genomics.org.cn) |
| 英文摘要 | The u-box domain proteins, with similar configuration to ring finger proteins, are highly conserved among eukaryotic organisms and most of them belong to the ubiquitin/proteasome system as e3 ubiquitin protein ligases, u-box containing proteins play a key role in the recognition and selection of abnormal proteins targeted for ubiquitination and subsequent degradation, a process for the maintenances and quality control of proteins exist in living cells. there are 77 u-box genes in rice genome and the systematic investigation of their expression will provide basic information for the functional analysis. the specific antibodies against rice u-box proteins was prepared to investigate the expression profile of u-box proteins at different developmental stages and accumulate basic information for functional studies. four rice u-box genes were chosen as their u-box domains are located at the n-terminal and posses arm repeats at c-terminal. epitopes prediction were carried out by computer software and the target protein fragments were expressed and purified in e. colt. system. polyclonal antibodies were generated by rabbit immunization. western blotting analysis were carried out for rice material collected at different developmental stages including shoot and root at seedling stage, root and stem at tilling stage, flag leaf and young particle at heading stage, flag leaf and particle at flowering stage, flag leaf and seed at filling stage. comparison analysis was carried out with est sequencing data. specific antibodies were obtained by rabbit immunization of recombinant proteins expressed in e. coli. one major band were observed for western blotting detection of rice u-box proteins, the apparent molecular mass of two u-box proteins (os06g01304 and os12g38210) were consistent with predicted size, while the other two proteins (os01g66130 and os08g01900) with apparent molecular mass smaller than that of predicted. western blotting results indicated that the u-box proteins were constitutively expressed with close abundance in tested tissues. est analysis based on i million ests derived from 274 libraries from ncbi est database revealed closed numbers of u-box gene transcription, which is parallel with the results of constitutively expression of proteins. however, compared with the expression of atpase, hsp81-3, egf-1 alpha and rubisco, the number of ests for u-box proteins were much lower, suggest the low abundance transcription of u-box genes. four rice u-box proteins were chosen for the generation of specific antibodies via the expression of predicted epitope fragments, demonstrated the feasibility of the process. western blotting analysis indicated that four u-box proteins were constitutively expressed among tested rice tissues at different developmental stages, which is parallel with est sequencing data. the antibodies will provide resources for functional studies, such as co-immunoprecipitation, chip-on-chip, pull-down and stress response etc. |
| WOS关键词 | E3 UBIQUITIN LIGASE ; ARABIDOPSIS ; STRESS ; DOMAIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000270102300017 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2401031 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu Guo-Zhen |
| 作者单位 | 1.Agr Univ Hebei, Coll Life Sci, Baoding 071001, Peoples R China 2.Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Hao,Li Li-Yun,Bai Hui,et al. Expression analysis of rice u-box proteins at different developmental stages[J]. Progress in biochemistry and biophysics,2009,36(9):1208-1214. |
| APA | Chen Hao,Li Li-Yun,Bai Hui,Cao Ying-Hao,Wang Xian-Yun,&Liu Guo-Zhen.(2009).Expression analysis of rice u-box proteins at different developmental stages.Progress in biochemistry and biophysics,36(9),1208-1214. |
| MLA | Chen Hao,et al."Expression analysis of rice u-box proteins at different developmental stages".Progress in biochemistry and biophysics 36.9(2009):1208-1214. |
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来源：中国科学院大学
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