中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Conserved mirna analysis in gossypium hirsutum through small rna sequencing

文献类型:期刊论文

作者Ruan, Meng-Bin2; Zhao, Ying-Tao1,3; Meng, Zhao-Hong2; Wang, Xiu-Jie1; Yang, Wei-Cai2
刊名Genomics
出版日期2009-10-01
卷号94期号:4页码:263-268
关键词Gossypium hirsutum Mirna Identification Sequencing
ISSN号0888-7543
DOI10.1016/j.ygeno.2009.07.002
通讯作者Wang, xiu-jie(xjwang@genetics.ac.cn)
英文摘要Several mirna family and their targets in cotton had been identified by computational methods based on the conserved characterization of mirnas. so far, there are no experiments to validate the existence of mirnas in cotton. in this study, to analyze the mirnas in cotton, a small rna library of sequences from 18 to 26 nt of gossypium hirsutum seedling has been built by high-throughput sequencing. in this library, 34 conserved mirna families were identified by homology search and the mirna* sequences of them were also found in the library. furthermore, potential targets of these conserved mirna families were predicted in cotton tc library. however, based on the mature mirnas and their mir* sequences, only 8 conserved mirna encoding loci (mir156, mir157a, mir157b, mir162, mir164, mir393, mir399, mir827) were identified from cotton est sequences. multiple encoding loci of some mirnas were identified by comparing the cloned mirna and mir* sequences. (c) 2009 elsevier inc. all rights reserved.
WOS关键词LATERAL ROOT DEVELOPMENT ; MESSENGER-RNA ; LEAF POLARITY ; CELL FATE ; MICRORNA ; PLANT ; ARABIDOPSIS ; IDENTIFICATION ; COTTON ; EVOLUTION
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目Biotechnology & Applied Microbiology ; Genetics & Heredity
语种英语
WOS记录号WOS:000273387100007
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
URI标识http://www.irgrid.ac.cn/handle/1471x/2401049
专题中国科学院大学
通讯作者Wang, Xiu-Jie
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Gen, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Key Lab Mol & Dev Biol, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Ruan, Meng-Bin,Zhao, Ying-Tao,Meng, Zhao-Hong,et al. Conserved mirna analysis in gossypium hirsutum through small rna sequencing[J]. Genomics,2009,94(4):263-268.
APA Ruan, Meng-Bin,Zhao, Ying-Tao,Meng, Zhao-Hong,Wang, Xiu-Jie,&Yang, Wei-Cai.(2009).Conserved mirna analysis in gossypium hirsutum through small rna sequencing.Genomics,94(4),263-268.
MLA Ruan, Meng-Bin,et al."Conserved mirna analysis in gossypium hirsutum through small rna sequencing".Genomics 94.4(2009):263-268.

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来源:中国科学院大学

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