Dynamic changes of hbv quasispecies and deletion patterns in a chronic hepatitis b patient
文献类型：期刊论文
| 作者 | Ji, Fen1,2; Zhou, Li3; Ma, Sufang1,2; Li, Feifei1,2; Ding, Huiguo3; Zeng, Changqing1 |
| 刊名 | Journal of medical virology
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 81期号:9页码:1551-1559 |
| 关键词 | Hepatitis b virus Lamivudine Adefovir dipivoxil Epitope |
| ISSN号 | 0146-6615 |
| DOI | 10.1002/jmv.21595 |
| 通讯作者 | Zeng, changqing(czeng@genomics.org.cn) |
| 英文摘要 | Hepatitis b virus (hbv) infection is a dynamic process during which molecular variants are selected continuously to adapt to changes. in addition to drug resistant mutations, sequential antiviral therapy may also lead to the selection of deleted mutants. to investigate this process, the following samples were collected from a patient who failed lamivudine therapy and then was switched to adefovir dipivoxil. hbv dna was sequenced at two separate regions; a 1 kb region of reverse transcriptase (rt) and a 1.5 kb region encompassing the c gene and part of the pres gene. sequence analysis of the rt region showed that the prevailing lamivudine resistant mutations were reduced after switching to adefovir dipivoxil, and ultimately the mutations were undetectable. quasispecies distribution and deletion patterns in the c and pres regions were also different between the two antiviral therapies. in lamivudine-treated samples, wildtype strains (57.7%) were dominant and deletions in the pres region were observed. however, in the subsequent therapy involving adefovir dipivoxil, a virus population harboring 81 and 96 bp deletions (86%) in the c gene prevailed. both major deletions encompassed t- and b-cell epitopes. meanwhile, the frequencies of the pres deletions decreased significantly, except for the 129 bp deletion. notably, the presence of 81, 96, and 129 bp deletions was always accompanied with some nucleotide substitutions. in conclusion, the prevalence of deletions at the c gene epitopes accompanied with the gradual disappearance of lamivudine resistance mutations may contribute to the survival of hbv under sequential antiviral therapy. j. med. virol. 81:1551-1559, 2009. (c) 2009 wiley-liss, inc. |
| WOS关键词 | ADEFOVIR DIPIVOXIL ; CORE-GENE ; HEPATOCELLULAR-CARCINOMA ; PRES DELETIONS ; VIRUS ; RESISTANT ; INFECTION ; LAMIVUDINE ; LIVER ; THERAPY |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000268555000007 |
| 出版者 | WILEY-LISS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2401067 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zeng, Changqing |
| 作者单位 | 1.Chinese Acad Sci, Beijing Inst Genom, Beijing 101300, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 101300, Peoples R China 3.Capital Med Univ, Beijing Youan Hosp, Beijing 100069, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Fen,Zhou, Li,Ma, Sufang,et al. Dynamic changes of hbv quasispecies and deletion patterns in a chronic hepatitis b patient[J]. Journal of medical virology,2009,81(9):1551-1559. |
| APA | Ji, Fen,Zhou, Li,Ma, Sufang,Li, Feifei,Ding, Huiguo,&Zeng, Changqing.(2009).Dynamic changes of hbv quasispecies and deletion patterns in a chronic hepatitis b patient.Journal of medical virology,81(9),1551-1559. |
| MLA | Ji, Fen,et al."Dynamic changes of hbv quasispecies and deletion patterns in a chronic hepatitis b patient".Journal of medical virology 81.9(2009):1551-1559. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。