Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase
文献类型：期刊论文
| 作者 | Guo, Zhimou1; Jin, Yu1,2; Liang, Tu1; Liu, Yanfang1; Xu, Qing1; Liang, Xinmiao1,2; Lei, Aiwen3 |
| 刊名 | Journal of chromatography a
 |
| 出版日期 | 2009-01-09 |
| 卷号 | 1216期号:2页码:257-263 |
| 关键词 | Beta-cyclodextrin Stationary phase Click chemistry Hydrophilic interaction liquid chromatography Polar components Mixed-mode stationary phase |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2008.11.071 |
| 通讯作者 | Liang, xinmiao(liangxm@dicp.ac.cn) |
| 英文摘要 | A native beta-cyclodextrin (beta-cd) stationary phase was prepared by covalently bonding beta-cd on silica particles via huisgen [3 + 2] dipolar cycloaddition between the organic azide and terminal alkyne, the so-called click chemistry. the resulting beta-cd bonded silica (click beta-cd) was characterized by ft-ir, solid state c-13 cross polarization/magic-angle spinning (cp/mas) nmr and elemental analyses. which proved the successful immobilization of beta-cd on the silica support with click chemistry. the retentive properties of click beta-cid were investigated under hydrophilic interaction liquid chromatography (hilic) mode in different mobile phase conditions with a set of polar compounds including nucleosides, organic acids and alkaloids. the effects of water content, concentration of the salt and ph of the buffer solution on the retention time were studied and the results demonstrated the typical retention behavior of hilic on click beta-cd. separation of very polar components, such as nucleosides and oligosaccharides, and chiral separation under hilic mode were successfully achieved. in addition, click beta-cd was chromatographically evaluated with a set of flavone glycosides. the retention curves depending on the mobile phase of acetonitrile content were "u" curves. which is an indication of hilic/rplc mixed-mode retention behavior. the difference of the separation selectivity between hilic and rplc was described as orthogonality by using geometric approach and the orthogonality reached 69.4%. the mixed-mode hplc properties and excellent orthogonality demonstrated the flexibility in hplc methods development and great potential in two-dimensional liquid chromatography separation. (c) 2008 elsevier b.v. all rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; POLAR COMPOUNDS ; SILICA-GEL ; MASS-SPECTROMETRY ; REVERSED-PHASE ; ADRENORECEPTOR AGONISTS ; RETENTION PREDICTION ; SEPARATION MATERIALS ; CHEMISTRY ; HPLC |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000262697100010 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2401068 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liang, Xinmiao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China 3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Zhimou,Jin, Yu,Liang, Tu,et al. Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase[J]. Journal of chromatography a,2009,1216(2):257-263. |
| APA | Guo, Zhimou.,Jin, Yu.,Liang, Tu.,Liu, Yanfang.,Xu, Qing.,...&Lei, Aiwen.(2009).Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase.Journal of chromatography a,1216(2),257-263. |
| MLA | Guo, Zhimou,et al."Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase".Journal of chromatography a 1216.2(2009):257-263. |
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来源：中国科学院大学
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