Molecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormone
文献类型：期刊论文
| 作者 | Hu, Wei; Chen, Xia; Yu, Gusong; Chen, Yan; Shen, Yan; Zhang, Ying; Gong, Yi |
| 刊名 | Protein and peptide letters
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 16期号:3页码:320-323 |
| 关键词 | Cell proliferation Cho-k1 cell Grass carp Growth hormone level Growth hormone receptor |
| ISSN号 | 0929-8665 |
| 通讯作者 | Gong, yi(yigong@srcb.ac.cn) |
| 英文摘要 | A novel grass carp growth hormone receptor (gcghr) cdna was cloned by rt-pcr method. cho-k1 cells were transfected with eukaryotic expression vector pcdna3.0 containing gcghr entire coding region, resulting in a stable cho cell line expressing gcghr (cho-gcghr). bioactivity assay revealed that silver carp growth hormone (scgh) bound gcghr with dissociation constant (k(d)) value of 1.96 nm and stimulated cho-gcghr cells proliferation with the sensitivity of 20 ng/ml. the cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein. |
| WOS关键词 | SIGNAL-TRANSDUCTION ; EXPRESSION ; SEABREAM ; FORMS ; FISH |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263980400016 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2401120 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gong, Yi |
| 作者单位 | Chinese Acad Sci, Grad Univ, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Wei,Chen, Xia,Yu, Gusong,et al. Molecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormone[J]. Protein and peptide letters,2009,16(3):320-323. |
| APA | Hu, Wei.,Chen, Xia.,Yu, Gusong.,Chen, Yan.,Shen, Yan.,...&Gong, Yi.(2009).Molecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormone.Protein and peptide letters,16(3),320-323. |
| MLA | Hu, Wei,et al."Molecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormone".Protein and peptide letters 16.3(2009):320-323. |
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来源：中国科学院大学
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