Linear ordered collagen scaffolds loaded with collagen-binding brain-derived neurotrophic factor improve the recovery of spinal cord injury in rats
文献类型：期刊论文
| 作者 | Han, Qianqian1,2; Sun, Wenjie1; Lin, Hang1; Zhao, Wenxue1; Gao, Yuan1; Zhao, Yannan1; Chen, Bing1; Xiao, Zhifeng1; Hu, Wei3; Li, Yuan3 |
| 刊名 | Tissue engineering part a
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 15期号:10页码:2927-2935 |
| ISSN号 | 1937-3341 |
| DOI | 10.1089/ten.tea.2008.0506 |
| 通讯作者 | Dai, jianwu(jwdai@genetics.ac.cn) |
| 英文摘要 | Growth factors such as brain-derived neurotrophic factor (bdnf) are widely used in the recovery of spinal cord injury (sci) for promoting axonal regeneration or improving neuron survival. however, the lack of efficient delivery approaches limits their clinical applications. in this study, we constructed an efficient delivery system including collagen-binding bdnf and linear ordered collagen scaffolds (locs). a collagen-binding domain (cbd) was utilized to construct collagen-targeting bdnf (cbd-bdnf) to allow its specific binding to the collagen. in vitro activity assay showed that cbd-bdnf had similar bioactivity in neurite outgrowth of dorsal root ganglia and the survival of pc12 cells. cbd-bdnf was shown to have specific binding activity to collagen. using the rat hemisection sci model, we found that locs loaded with cbd-bdnf significantly improved the sci recovery evaluated by the basso, beattie, and bresnahan scale and immunohistochemical staining with anti-neurofilament antibody. thus, this targeting drug delivery system consisting of cbd-bdnf and locs could be an effective strategy for the repair of sci. |
| WOS关键词 | DORSAL-ROOT GANGLION ; GROWTH-FACTOR ; BDNF ; REGENERATION ; NEURONS ; CELLS ; NT-3 ; SURVIVAL ; DELIVERY ; REPAIR |
| WOS研究方向 | Cell Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000270553200015 |
| 出版者 | MARY ANN LIEBERT, INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2401759 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dai, Jianwu |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Mol Dev Biol, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Zhengzhou Univ, Dept Neurosurg, Affiliated Hosp 1, Zhengzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Qianqian,Sun, Wenjie,Lin, Hang,et al. Linear ordered collagen scaffolds loaded with collagen-binding brain-derived neurotrophic factor improve the recovery of spinal cord injury in rats[J]. Tissue engineering part a,2009,15(10):2927-2935. |
| APA | Han, Qianqian.,Sun, Wenjie.,Lin, Hang.,Zhao, Wenxue.,Gao, Yuan.,...&Dai, Jianwu.(2009).Linear ordered collagen scaffolds loaded with collagen-binding brain-derived neurotrophic factor improve the recovery of spinal cord injury in rats.Tissue engineering part a,15(10),2927-2935. |
| MLA | Han, Qianqian,et al."Linear ordered collagen scaffolds loaded with collagen-binding brain-derived neurotrophic factor improve the recovery of spinal cord injury in rats".Tissue engineering part a 15.10(2009):2927-2935. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。