Enhanced tolerance to chilling stress in osmyb3r-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes
文献类型：期刊论文
| 作者 | Ma, Qibin1,2; Dai, Xiaoyan1; Xu, Yunyuan1,4; Guo, Jing3; Liu, Yaju1,2; Chen, Na1,2; Xiao, Jun1,2; Zhang, Dajian1,2; Xu, Zhihong1,4; Zhang, Xiansheng3 |
| 刊名 | Plant physiology
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 150期号:1页码:244-256 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.108.133454 |
| 通讯作者 | Chong, kang(chongk@ibcas.ac.cn) |
| 英文摘要 | Myb transcription factors play central roles in plant responses to abiotic stresses. how stress affects development is poorly understood. here, we show that osmyb3r-2 functions in both stress and developmental processes in rice (oryza sativa). transgenic plants overexpressing osmyb3r-2 exhibited enhanced cold tolerance. cold treatment greatly induced the expression of osmyb3r-2, which encodes an active transcription factor. we show that osmyb3r-2 specifically bound to a mitosis-specific activator cis-element, (t/c) c(t/c) aacgg(t/c)(t/c) a, a conserved sequence that was found in promoters of cyclin genes such as oscycb1;1 and osknolle2. in addition, overexpression of osmyb3r-2 in rice led to higher transcript levels of several g2/m phase-specific genes, including oscycb1;1, oscycb2;1, oscycb2;2, and oscdc20.1, than those in osmyb3r-2 antisense lines or wild-type plants in response to cold treatment. flow cytometry analysis revealed an increased cell mitotic index in overexpressed transgenic lines of osmyb3r-2 after cold treatment. furthermore, resistance to cold stress in the transgenic plants overexpressing oscycb1;1 was also enhanced. the level of cellular free proline was increased in the overexpressed rice lines of osmyb3r-2 and oscycb1;1 transgenic plants compared with wild-type plants under the cold treatment. these results suggest that osmyb3r-2 targets oscycb1;1 and regulates the progress of the cell cycle during chilling stress. oscpt1, which may be involved in the dehydration-responsive element-binding factor 1a pathway, showed the same transcription pattern in response to cold as did oscycb1;1 in transgenic rice. therefore, a cold resistance mechanism in rice could be mediated by regulating the cell cycle, which is controlled by key genes including osmyb3r-2. |
| WOS关键词 | ORYZA-SATIVA L. ; TRANSCRIPTION FACTOR GENE ; CA2+-DEPENDENT PROTEIN-KINASE ; ZINC-FINGER PROTEIN ; C-MYB ; COLD-STRESS ; ARABIDOPSIS-THALIANA ; FREEZING TOLERANCE ; DROUGHT TOLERANCE ; SALT-STRESS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000265661700021 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2401800 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chong, Kang |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Res Ctr Mol & Dev Biol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China 4.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Qibin,Dai, Xiaoyan,Xu, Yunyuan,et al. Enhanced tolerance to chilling stress in osmyb3r-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes[J]. Plant physiology,2009,150(1):244-256. |
| APA | Ma, Qibin.,Dai, Xiaoyan.,Xu, Yunyuan.,Guo, Jing.,Liu, Yaju.,...&Chong, Kang.(2009).Enhanced tolerance to chilling stress in osmyb3r-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes.Plant physiology,150(1),244-256. |
| MLA | Ma, Qibin,et al."Enhanced tolerance to chilling stress in osmyb3r-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes".Plant physiology 150.1(2009):244-256. |
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来源：中国科学院大学
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