A novel tyrosinase biosensor based on biofunctional zno nanorod microarrays on the nanocrystalline diamond electrode for detection of phenolic compounds
文献类型：期刊论文
| 作者 | Zhao, Jianwen1; Wu, Daohong1; Zhi, Jinfang2,3 |
| 刊名 | Bioelectrochemistry
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 75期号:1页码:44-49 |
| 关键词 | Tyrosinase Boron-doped nanocrystalline diamond thin film Biofunctional zno nanorod microarrays Phenolic compounds |
| ISSN号 | 1567-5394 |
| DOI | 10.1016/j.bioelechem.2009.01.005 |
| 通讯作者 | Zhao, jianwen(zhjw76@hotmail.com) |
| 英文摘要 | A novel tyrosinase biosensor based on biofuncational zno nanorod microarrays on the boron-doped nanocrystalline diamond (bdnd) substrates was developed. the zno nanorod microarrays were firstly deposited on bdnd thin film surfaces via a low-temperature solution method, and then zno nanorods were functionalized with the mixture of 3-aminopropyltriethoxysilane (aptes) and tetraethoxysilane (teos) by a co-condensation approach, then tyrosinase was immobilized to amino-modification zno nanorod surfaces by the covalent binding. as-prepared tyrosinase biosensors were used for the detection of phenolic compounds. the tyrosinase-modified bdnd electrode gave a linear response range of 1-175,1-150 and 1-150 mu m and sensitivity of 576.2, 339.3 and 287.1 mu a mmol(-1) cm(-2) for p-cresol, 4-chlorophenol and phenol, respectively. the low detection limit was estimated to be 0.1, 0.25 and 0.2 mu m (s(b)/m = 3), respectively. therefore, the biolfunctional zno nanorod arrays have potential applications as platforms to immobilize other enzymes and bioactive molecules in biosensors. (c) 2009 elsevier b.v. all rights reserved. |
| WOS关键词 | POLYCRYSTALLINE DIAMOND ; IMMOBILIZATION ; FILMS ; ARRAYS ; NANOPARTICLES ; FABRICATION ; GLUCOSE ; MATRIX ; SENSOR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Biophysics ; Electrochemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Biology ; Biophysics ; Electrochemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000265111500008 |
| 出版者 | ELSEVIER SCIENCE SA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2402396 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, Jianwen |
| 作者单位 | 1.Jishou Univ, Coll Chem & Chem Engn, Jishou 416000, Hunan, Peoples R China 2.Chinese Acad Sci, Tech Inst Phys & Chem, Lab Organ Optoelect Funct Mat & Mol Engn, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jianwen,Wu, Daohong,Zhi, Jinfang. A novel tyrosinase biosensor based on biofunctional zno nanorod microarrays on the nanocrystalline diamond electrode for detection of phenolic compounds[J]. Bioelectrochemistry,2009,75(1):44-49. |
| APA | Zhao, Jianwen,Wu, Daohong,&Zhi, Jinfang.(2009).A novel tyrosinase biosensor based on biofunctional zno nanorod microarrays on the nanocrystalline diamond electrode for detection of phenolic compounds.Bioelectrochemistry,75(1),44-49. |
| MLA | Zhao, Jianwen,et al."A novel tyrosinase biosensor based on biofunctional zno nanorod microarrays on the nanocrystalline diamond electrode for detection of phenolic compounds".Bioelectrochemistry 75.1(2009):44-49. |
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来源：中国科学院大学
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