Functional characterization of pgrp-lc1 of anopheles gambiae through deletion and rna interference
文献类型：期刊论文
| 作者 | Chen, Yang1,2; Ling, Erjun1; Weng, Zhihui1 |
| 刊名 | Insect science
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 16期号:6页码:443-453 |
| 关键词 | Antimicrobial peptide Malaria Mosquito Peptidoglycan recognition protein Rnai |
| ISSN号 | 1672-9609 |
| DOI | 10.1111/j.1744-7917.2009.01296.x |
| 通讯作者 | Chen, yang(philchan@126.com) |
| 英文摘要 | Peptidoglycan recognition proteins (pgrp) play an important role in innate immunity in insects through the activation of the imd pathway, which has been shown to be required in the antibacterial response in insects and in the limitation of the number of plasmodium berghei oocysts developing in mosquito midgut. the lc1 gene of the prgp family in anopheles gambiae produces many products through alternative splicing. in this work, we demonstrate that pgrp-lc1a alone is sufficient to activate the imd pathway in the a. gambiae l3-5 cell line through a combination of terminal or internal deletions, and rna interference against endogenous pgrp-lc products. in the absence of endogenous pgrp-lc proteins, the integrity of the cytoplasmic domain is necessary for lc1a function, while that of the extracellular domain is not. moreover, the shorter the extracellular domain, the higher the activity for lc1a. however, the removal of either the cytoplasmic or the extracellular pgrp-binding domain has little impact on the activity of lc1a in the presence of endogenous pgrp-lc proteins. |
| WOS关键词 | PEPTIDOGLYCAN RECOGNITION PROTEIN ; DROSOPHILA IMMUNE-RESPONSE ; NEGATIVE REGULATOR ; IMD-PATHWAY ; BLOOD MEAL ; IN-VITRO ; LC ; GENES ; INFECTION ; MELANOGASTER |
| WOS研究方向 | Entomology |
| WOS类目 | Entomology |
| 语种 | 英语 |
| WOS记录号 | WOS:000272194500002 |
| 出版者 | WILEY-BLACKWELL PUBLISHING, INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2402957 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Yang |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Insect Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yang,Ling, Erjun,Weng, Zhihui. Functional characterization of pgrp-lc1 of anopheles gambiae through deletion and rna interference[J]. Insect science,2009,16(6):443-453. |
| APA | Chen, Yang,Ling, Erjun,&Weng, Zhihui.(2009).Functional characterization of pgrp-lc1 of anopheles gambiae through deletion and rna interference.Insect science,16(6),443-453. |
| MLA | Chen, Yang,et al."Functional characterization of pgrp-lc1 of anopheles gambiae through deletion and rna interference".Insect science 16.6(2009):443-453. |
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来源：中国科学院大学
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