Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film
文献类型：期刊论文
| 作者 | Zheng, Na; Zhou, Xia; Yang, Weiying; Li, Xiangjun; Yuan, Zhuobin |
| 刊名 | Talanta
 |
| 出版日期 | 2009-08-15 |
| 卷号 | 79期号:3页码:780-786 |
| 关键词 | Fe(3)o(4) nanoparticles Direct electrochemistry Electrocatalysis Hemoglobin Hydrogen peroxide |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2009.05.002 |
| 通讯作者 | Li, xiangjun(lixiangj@gucas.ac.cn) |
| 英文摘要 | Magnetic nanoparticles (fe(3)o(4)) were synthesized by a chemical coprecipitation method. x-ray diffraction (xrd) and transmission electron microscope (tem) were used to confirm the crystallite structure and the particle's radius. the fe(3)o(4) nanoparticles and chitosan (cs) were mixed to form a matrix in which haemoglobin (hb) can be immobilized for the fabrication of h(2)o(2) biosensor. the fe(3)o(4)-cs-hb film exhibited a pair of well-defined and quasi-reversible cyclic voltammetric peaks due to the redox of hb-heme fe (iii)/fe (ii) in a ph 7.0 phosphate buffer. the formal potential of hb-heme fe(iii)/fe(ii) couple varied linearly with the increase of ph in the range of 4.0-10.0 with a slope of 46.5 mv ph(-1), indicating that electron transfer was accompanied with single proton transportation in the electrochemical reaction. the surface coverage of hb immobilized on fe(3)o(4)-cs film glassy carbon electrode was about 1.13 x 10(-10) mol cm(-2). the heterogeneous electron transfer rate constant(k(s)) was 1.04 s(-1), indicating great facilitation of the electron transfer between hb and magnetic nanoparticles-chitosan modified electrode. the modified electrode showed excellent electrocatalytic activity toward oxygen and hydrogen peroxide reduction. the apparent michaelis-menten constant k(m)(app) for h(2)o(2) was estimated to be 38.1 mu mol l(-1). (c) 2009 elsevier b.v. all rights reserved. |
| WOS关键词 | DIRECT ELECTRON-TRANSFER ; MODIFIED GOLD ELECTRODE ; GLUCOSE-OXIDASE ; CARBON NANOTUBE ; PROTEIN ; BIOELECTROCHEMISTRY ; VOLTAMMETRY ; PARTICLES ; COMPOSITE ; BIOSENSOR |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000268346800034 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2402982 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Xiangjun |
| 作者单位 | Chinese Acad Sci, Grad Univ, Coll Chem & Chem Engn, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Na,Zhou, Xia,Yang, Weiying,et al. Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film[J]. Talanta,2009,79(3):780-786. |
| APA | Zheng, Na,Zhou, Xia,Yang, Weiying,Li, Xiangjun,&Yuan, Zhuobin.(2009).Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film.Talanta,79(3),780-786. |
| MLA | Zheng, Na,et al."Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film".Talanta 79.3(2009):780-786. |
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来源：中国科学院大学
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