Prostasin regulates inos and cyclin d1 expression by modulating protease-activated receptor-2 signaling in prostate epithelial cells
文献类型：期刊论文
| 作者 | Chen, Li-Mei1; Hatfield, Meghan L.1; Fu, Ya-Yuan2,3; Chai, Karl X.1,4 |
| 刊名 | Prostate
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 69期号:16页码:1790-1801 |
| 关键词 | Prss8 Cap-1 Glycosylphosphatidylinositol anchor |
| ISSN号 | 0270-4137 |
| DOI | 10.1002/pros.21030 |
| 通讯作者 | Chai, karl x.(kxchai@mail.ucf.edu) |
| 英文摘要 | Background. prostasin is down-regulated during inflammation and in invasive cancers, and plays a role in regulation of inflammatory gene expression and invasion. methods. we used the human benign prostatic hyperplasia cell line bph-1 to investigate gene expression changes associated with sirna-mediated loss of prostasin expression. quantitative pcr and/or western blotting were used to evaluate the expression changes of inos, icam-1, cyclin d1, il-6, and il-8. gene expression changes were also evaluated in the presence of a par-2 antagonist. the pc-3 human prostate cancer cell line was used for evaluation of gene expression in response to prostasin re-expression. results. prostasin silencing in bph-1 was associated with up-regulation of inos, icam-1, il-6, and il-8, and down-regulation of cyclin d1; as well as reduced proliferation and invasion. the inos up-regulation and cyclin d1 down-regulation associated with prostasin silencing were inhibited by a par-2 antagonist. re-expression of prostasin, a serine active-site mutant, and a gpi-anchor-free mutant, in the pc-3 cells resulted in par-2 and cyclin d1 transcription up-regulation. transcription up-regulation of il-6 and il-8 was associated with re-expression of the serine active-site mutant prostasin in the pc-3 cells. transcription up-regulation of il-8, but to a lesser extent, was also observed in pc-3 cells expressing the wild-type prostasin. expression of a serine protease active prostasin, gpi-anchored or anchor-free, prevented the il-6 induction in response to par-2. the gpi-anchor-free prostasin also prevented the il-8 induction. conclusions. prostasin plays a negative regulatory role on par-2-mediated signaling in prostate epithelial cells. prostate 69: 1790-1801, 2009. (c) 2009 wiley-liss, inc. |
| WOS关键词 | NITRIC-OXIDE SYNTHASE ; SERINE-PROTEASE ; BREAST-CANCER ; INTERLEUKIN-8 PRODUCTION ; ENDOTHELIAL-CELLS ; PAR-2 ACTIVATION ; DNA METHYLATION ; PROMOTER DNA ; CARCINOMA ; LINE |
| WOS研究方向 | Endocrinology & Metabolism ; Urology & Nephrology |
| WOS类目 | Endocrinology & Metabolism ; Urology & Nephrology |
| 语种 | 英语 |
| WOS记录号 | WOS:000272312400011 |
| 出版者 | WILEY-LISS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2403633 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Karl X. |
| 作者单位 | 1.Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Dept Mol Biol & Microbiol, Orlando, FL 32816 USA 2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Biomol Sci Ctr, Orlando, FL 32816 USA |
| 推荐引用方式 GB/T 7714 | Chen, Li-Mei,Hatfield, Meghan L.,Fu, Ya-Yuan,et al. Prostasin regulates inos and cyclin d1 expression by modulating protease-activated receptor-2 signaling in prostate epithelial cells[J]. Prostate,2009,69(16):1790-1801. |
| APA | Chen, Li-Mei,Hatfield, Meghan L.,Fu, Ya-Yuan,&Chai, Karl X..(2009).Prostasin regulates inos and cyclin d1 expression by modulating protease-activated receptor-2 signaling in prostate epithelial cells.Prostate,69(16),1790-1801. |
| MLA | Chen, Li-Mei,et al."Prostasin regulates inos and cyclin d1 expression by modulating protease-activated receptor-2 signaling in prostate epithelial cells".Prostate 69.16(2009):1790-1801. |
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来源：中国科学院大学
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