Study of the biosurfactant-producing profile in a newly isolated rhodococcus ruber strain
文献类型:期刊论文
作者 | Zheng, Chenggang1; Li, Shuguang2; Yu, Li3; Huang, Lixin3; Wu, Qinghong3 |
刊名 | Annals of microbiology
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出版日期 | 2009 |
卷号 | 59期号:4页码:771-776 |
关键词 | Rhodococcus ruber Biomass Emulsification index Cellular hydrophobicity Biosurfactant-producing profile |
ISSN号 | 1590-4261 |
通讯作者 | Zheng, chenggang(cg1982.zheng@gmail.com) |
英文摘要 | A biosurfactant-producing strain z25 was isolated from the oil production water in daqing oilfield, china. the strain was identified as rhodococcus ruber by 16s rdna sequencing. rhodococcus ruber z25 showed a preference of alkanes as carbon sources for biosurfactant synthesis. the optimal biosurfactant production was achieved at the nacl concentration of 2.5% at 34 degrees c. in batch cultivation, r. ruber z25 exhibited a cell-growth associated biosurfactant-producing process on n-hexadecane and the maximum yield of biosurfactant production was 13.34 g/l at 44 h. the rate of biosurfactant production per unit biomass (r-(biosurf/biomass)) was used to characterize the biosurfactant-producing profile. a two-stage biosurfactant-producing profile of r. ruber z25 was established by the r-(biosurf/biomass) curve, exhibiting the biosurfactant production in correlation with cellular hydrophobicity and biomass accumulation. the crude biosurfactant was extracted by methyl tert-butyl ether (mtbe) method and only one glycolipid fraction at rf value of 0.64 was detected by thin layer chromatography (tlc) analysis. |
WOS关键词 | RESPONSE-SURFACE OPTIMIZATION ; MICROBIAL-PRODUCTION ; ERYTHROPOLIS ; BACTERIA |
WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000274263200020 |
出版者 | SPRINGER |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2404266 |
专题 | 中国科学院大学 |
通讯作者 | Zheng, Chenggang |
作者单位 | 1.Chinese Acad Sci, Grad Univ, Inst Porous Flow & Fluid Mech, Langfang 065007, Peoples R China 2.China Natl Petr Corp, Coal Bed Gas Co, Beijing 100081, Peoples R China 3.China Natl Petr Corp, Res Inst Petr Exploitat & Dev Langfang, Langfang 065007, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, Chenggang,Li, Shuguang,Yu, Li,et al. Study of the biosurfactant-producing profile in a newly isolated rhodococcus ruber strain[J]. Annals of microbiology,2009,59(4):771-776. |
APA | Zheng, Chenggang,Li, Shuguang,Yu, Li,Huang, Lixin,&Wu, Qinghong.(2009).Study of the biosurfactant-producing profile in a newly isolated rhodococcus ruber strain.Annals of microbiology,59(4),771-776. |
MLA | Zheng, Chenggang,et al."Study of the biosurfactant-producing profile in a newly isolated rhodococcus ruber strain".Annals of microbiology 59.4(2009):771-776. |
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来源:中国科学院大学
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