[ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor
文献类型：期刊论文
| 作者 | Hu, Lianzhe1,2; Bian, Zheng3; Li, Haijuan1,2; Han, Shuang1,2; Yuan, Yali1,2; Gao, Lianxun3; Xu, Guobao1 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 81期号:23页码:9807-9811 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac901925x |
| 通讯作者 | Xu, guobao(guobaoxu@ciac.jl.cn) |
| 英文摘要 | [ru(bpy)(2)dppz](2+) electrochemilumineseence (ecl) was studied, and it was used to investigate dna interaction and develop a label-free atp aptasensor for the first time. ecl of [ru(bpy)(2)dppz](2+) is negligible in aqueous solution, and increases similar to 1000 times when [ru(bpy)(2)dppz](2+) intercalates into the nucleic acid structure. the ecl switch behavior of [ru(bpy)(2)dppz](2+) is ascribed to the intercalation that shields the phenazine nitrogens from the solvent and results in a luminescent excited state. the ecl switch by dna was applied to investigate the interaction of [ru(bpy)(2)dppz](2+) with herring sperm dna. the calculated equilibrium constant (k) is 1.35 x 10(6) m-1, and the calculated binding-site size (s) is 0.88 base pair, which is consistent with the reported values. moreover, atp can dramatically affect ecl of the [ru(bpy)(2)dppz](2+)/atp aptamer complex. as a result, a label-free, sensitive, and selective [ru(bpy)(2)dppz](2+) ecl method for atp detection was developed. the detection limit is 100 nm for atp (with a signal-to-noise ratio, s/n, of 3) with a linear range of 0-1 mu m. ne result demonstrates that [ru(bpy)(2)dppz](2+) ecl holds great promise in aptasensors. |
| WOS关键词 | MOLECULAR LIGHT SWITCH ; ELECTROGENERATED CHEMILUMINESCENCE ; DIPYRIDOPHENAZINE COMPLEXES ; APTAMER ; RUTHENIUM ; AMPLIFICATION ; SENSITIVITY ; BINDING ; SYSTEM ; ECL |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000272084700043 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2404596 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, Guobao |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Lianzhe,Bian, Zheng,Li, Haijuan,et al. [ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor[J]. Analytical chemistry,2009,81(23):9807-9811. |
| APA | Hu, Lianzhe.,Bian, Zheng.,Li, Haijuan.,Han, Shuang.,Yuan, Yali.,...&Xu, Guobao.(2009).[ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor.Analytical chemistry,81(23),9807-9811. |
| MLA | Hu, Lianzhe,et al."[ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor".Analytical chemistry 81.23(2009):9807-9811. |
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来源：中国科学院大学
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