Simultaneous determination of 8 poisonous alkaloids in human blood by liquid chromatography-electrospray ionization tandem mass spectrometry
文献类型：期刊论文
| 作者 | Xiong Xiao-Ting1,2,3; Wu Hui-Qin1; Huang Xiao-Lan1 |
| 刊名 | Chinese journal of analytical chemistry
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 37期号:10页码:1433-1438 |
| 关键词 | Liquid chromatography Electrospray ionization tandem mass spectrometry Multiple-reaction monitoring Simultaneous determination Poisonous alkaloids Blood |
| ISSN号 | 0253-3820 |
| 通讯作者 | Wu hui-qin(wu_hui_qin@tom.com) |
| 英文摘要 | A novel method was developed and validated for the rapid and simultaneous determination of 8 poisonous alkaloids, ephedrine, pilocarpine, strychnine, atropine, brucine, aconitine, camptothecin, and koumine, in human blood using liquid chromatography-electrospray ionization tandem mass spectrometry in the multiple reaction monitoring(lc-esi-mrm) mode. the alkaloids in blood samples, treated with ammonium acetate-ammonia buffer solution of ph approximate to 9, were extracted by methanol. the conditions of extraction, chromatography and mass spectrometry were optimized. the linearity of first 4 compounds were held in the ranges of 1 - 100 mu g/l, while others were 2.5 - 500 mu g/l. the average extraction recoveries were in the range of 83.1% - 104.0%. the limits of detection(lod) and quantification(loq) of strychnine, atropine, brucine, aconitine, and koumine were 0.05 mu g/l and 0.1 mu g/l, respectively, while the other 3 poisonous were 0.1 mu g/l and 0.5 mu g/l, respectively. the intra-day relative standard deviation(rsd) was less than 9%, while the inter-day relative standard deviation was less than 10%. the method, proved to be simple, rapid, sufficiently selective and sensitive, was suitable for poisoning diagnosis and forensic toxicology analysis. |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000271686300006 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2404770 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wu Hui-Qin |
| 作者单位 | 1.China Natl Analyt Ctr, Guangdong Prov Key Lab Emergency Test Dangerous C, Guangzhou 510070, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Chem, Guangzhou 510650, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong Xiao-Ting,Wu Hui-Qin,Huang Xiao-Lan. Simultaneous determination of 8 poisonous alkaloids in human blood by liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Chinese journal of analytical chemistry,2009,37(10):1433-1438. |
| APA | Xiong Xiao-Ting,Wu Hui-Qin,&Huang Xiao-Lan.(2009).Simultaneous determination of 8 poisonous alkaloids in human blood by liquid chromatography-electrospray ionization tandem mass spectrometry.Chinese journal of analytical chemistry,37(10),1433-1438. |
| MLA | Xiong Xiao-Ting,et al."Simultaneous determination of 8 poisonous alkaloids in human blood by liquid chromatography-electrospray ionization tandem mass spectrometry".Chinese journal of analytical chemistry 37.10(2009):1433-1438. |
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来源：中国科学院大学
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