Phylogenetic diversity of dissimilatory ferric iron reducers in paddy soil of hunan, south china
文献类型：期刊论文
| 作者 | Wang, Xin-Jun1,2; Yang, Jing1; Chen, Xue-Ping1; Sun, Guo-Xin1; Zhu, Yong-Guan1,3 |
| 刊名 | Journal of soils and sediments
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 9期号:6页码:568-577 |
| 关键词 | Arsenic Dissimilatory iron-reducing bacteria Enrichment cultures Paddy soil Terminal restriction fragment length polymorphism (t-rflp) |
| ISSN号 | 1439-0108 |
| DOI | 10.1007/s11368-009-0113-x |
| 通讯作者 | Zhu, yong-guan(ygzhu@rcees.ac.cn) |
| 英文摘要 | Dissimilatory iron-reducing bacteria have been described by both culture-dependent and -independent methods in various environments, including freshwater, marine sediments, natural wetlands, and contaminated aquifers. however, little is known about iron-reducing microbial communities in paddy soils. the goal of this study was to characterize iron-reducing microbial communities in paddy soil. moreover, the effect of dissolved and solid-phase iron (iii) species on the iron-reducing microbial communities was also investigated by enrichment cultures. ferric citrate and ferrihydrite were used respectively to set up enrichment cultures of dissimilatory iron-reducing microorganisms using 1% inoculum of soil samples, and the iron reduction was measured. moreover, bacterial dna was extracted and 16s rrna genes were pcr-amplified, and subsequently analyzed by the clone library and terminal restriction fragment length polymorphism (t-rflp). phylogenetic analysis of 16s rrna gene sequences extracted from the enrichment cultures revealed that bradyrhizobium, bacteroides, clostridium and ralstonia species were the dominant bacteria in the ferric citrate enrichment. however, members of the genera clostridium, bacteroides, and geobacter were the dominant microorganisms in the ferrihydrite enrichment. analysis of enrichment cultures by t-rflp strongly supported the cloning and sequencing results. the present study demonstrated that dissimilatory iron-reducing consortia in as-contaminated paddy soil are phylogenetically diverse. moreover, iron (iii) sources as a key factor have a strong effect on the iron (iii)-reducing microbial community structure and relative abundance in the enrichments. in addition, geobacter species are selectively enriched by ferrihydrite enrichment cultures. |
| WOS关键词 | RICE-FIELD SOIL ; ANAEROBIC AMMONIUM OXIDATION ; SP-NOV. ; PLANT RESIDUE ; FE(III)-REDUCING BACTERIA ; RALSTONIA-METALLIDURANS ; REDUCING BACTERIA ; GEN. NOV. ; REDUCTION ; SEDIMENTS |
| WOS研究方向 | Geology ; Agriculture |
| WOS类目 | Geosciences, Multidisciplinary ; Soil Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000271643900007 |
| 出版者 | SPRINGER HEIDELBERG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2404900 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, Yong-Guan |
| 作者单位 | 1.Chinese Acad Sci, Sate Key Lab Urban & Reg Ecol, Ecoenvironm Sci Res Ctr, Beijing 10085, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Key Lab Urban Environm & Hlth, Inst Urban Environm, Xiamen 361021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xin-Jun,Yang, Jing,Chen, Xue-Ping,et al. Phylogenetic diversity of dissimilatory ferric iron reducers in paddy soil of hunan, south china[J]. Journal of soils and sediments,2009,9(6):568-577. |
| APA | Wang, Xin-Jun,Yang, Jing,Chen, Xue-Ping,Sun, Guo-Xin,&Zhu, Yong-Guan.(2009).Phylogenetic diversity of dissimilatory ferric iron reducers in paddy soil of hunan, south china.Journal of soils and sediments,9(6),568-577. |
| MLA | Wang, Xin-Jun,et al."Phylogenetic diversity of dissimilatory ferric iron reducers in paddy soil of hunan, south china".Journal of soils and sediments 9.6(2009):568-577. |
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来源：中国科学院大学
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