Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody
文献类型：期刊论文
| 作者 | Zeng, Qinghui1,2; Zhang, Youlin1,3; Song, Kai1; Kong, Xianggui1; Aalders, Maurice C. G.4; Zhang, Hong3 |
| 刊名 | Talanta
 |
| 出版日期 | 2009-11-15 |
| 卷号 | 80期号:1页码:307-312 |
| 关键词 | Cdte/cds core/shell quantum dots Protein g Flexible coupling Fluorescent immune detection Specificity |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2009.06.061 |
| 通讯作者 | Kong, xianggui(xgkong14@ciomp.ac.cn) |
| 英文摘要 | Quantum dots (qds) are widely used in the immune detection. yet, the sensitivity and specificity of the immune detection are not satisfactory because the binding sites of qds onto antibody (ab) are often arbitrary and the influence of the large surface electronic potential energy of qds on the directly conjugated ab is nonnegligible. in this work, we provide a "flexible" coupling method, in which protein g (pg) is selected as the flexible bridge between the qds and the hepatitis b virus surface antibody (hbsab), to improve the sensitivity and specificity of the fluoroimmunoassay compared to the directly covalent conjugation. successful coupling of the hbsab to our highly luminescent cdte/cds core/shell qds is proven with gel electrophoresis and atomic force microscopy (afm). the assay results, based on the microelisa well plate as matrix to immobilize the sandwich structure, show that both sensitivity and specificity can be improved greatly through the flexible coupled qds-pg-ab conjugates. (c) 2009 published by elsevier b.v. |
| WOS关键词 | ATOMIC-FORCE MICROSCOPY ; IN-VIVO ; NANOCRYSTALS ; PROTEIN ; CDTE ; CONJUGATION ; CDSE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000271055700046 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2404920 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Kong, Xianggui |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Opt Fine Mech & Phys, Key Lab Excited State Proc, Changchun 130033, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Amsterdam, Vant Hoff Inst Mol Sci, NL-1018 WV Amsterdam, Netherlands 4.Univ Amsterdam, Acad Med Ctr, Dept Biomed Engn & Phys, NL-1105 AZ Amsterdam, Netherlands |
| 推荐引用方式 GB/T 7714 | Zeng, Qinghui,Zhang, Youlin,Song, Kai,et al. Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody[J]. Talanta,2009,80(1):307-312. |
| APA | Zeng, Qinghui,Zhang, Youlin,Song, Kai,Kong, Xianggui,Aalders, Maurice C. G.,&Zhang, Hong.(2009).Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody.Talanta,80(1),307-312. |
| MLA | Zeng, Qinghui,et al."Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody".Talanta 80.1(2009):307-312. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。